A simplified and rapid test for acetylator phenotyping by use of the peak height ratio of two urinary caffeine metabolites.
We describe a simplified liquid-chromatographic test in which acetylator phenotype is determined by measuring the peak height ratio of two urinary caffeine metabolites, 5-acetylamino-6-formylamino-3-methyluracil and 1-methylxanthine. We applied this test to determine the acetylator phenotypes of 52 subjects who regularly drink coffee, tea, or caffeinated beverages. Also, we determined the acetylator phenotypes of these subjects according to a well-established sulfasalazine test, which yielded identical results. We established the reproducibility of the described test by determining the acetylator phenotypes of 10 additional subjects on two different days separated by a period of two to five weeks. Of the 52 subjects examined by both tests, 40 (76.9%) were classified as slow acetylators, which agrees well with the percentage reported elsewhere for 297 similar subjects from the Saudi population.[1]References
- A simplified and rapid test for acetylator phenotyping by use of the peak height ratio of two urinary caffeine metabolites. el-Yazigi, A., Chaleby, K., Martin, C.R. Clin. Chem. (1989) [Pubmed]
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