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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy.

We have determined the sequence of the E. coli gyrB gene, using a new sequencing approach in which transposition from a mini-Mu plasmid into the DNA provides random start points for dideoxynucleotide sequence analysis. The gyrB sequence corresponds to a protein 804 amino acids long; a previously isolated protein fragment with partial enzymatic activity has been identified as the C-terminal half-molecule. A plausible terminator of gyrB transcription is located just beyond the structural gene.[1]

References

  1. DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy. Adachi, T., Mizuuchi, M., Robinson, E.A., Appella, E., O'Dea, M.H., Gellert, M., Mizuuchi, K. Nucleic Acids Res. (1987) [Pubmed]
 
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