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Cloning, sequence, and expression of bovine interferon-gamma.

Bovine interferon-gamma (IFN-gamma) sequences have been isolated by screening a cDNA library with a human IFN-gamma cDNA probe. The cDNA library was constructed from RNA isolated from concanavalin A-stimulated bovine lymph node cells. The open reading frame predicts that the bovine IFN-gamma precursor is composed of 166 amino acids with a predicted m.w. of 19,393. Alignment of the amino acid sequence with human IFN-gamma indicates that mature bovine IFN-gamma is composed of 143 amino acids with a predicted m.w. of 16,858. It has an amino acid homology of 63% with human IFN-gamma, and 47% with murine IFN-gamma. Biologically active bovine IFN-gamma was synthesized in an Escherichia coli expression system.[1]


  1. Cloning, sequence, and expression of bovine interferon-gamma. Cerretti, D.P., McKereghan, K., Larsen, A., Cosman, D., Gillis, S., Baker, P.E. J. Immunol. (1986) [Pubmed]
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