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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Reutilization of 125I-labelled anti-IgE antibody and paper discs in PRIST and RAST IgE determination.

In the paper radioimmunosorbent test (PRIST) anti-human IgE coupled paper discs are used for the estimation of total IgE in blood serum: in the radioallergosorbent test (RAST) allergen-coupled paper discs are used for the estimation of specific IgE in blood serum. The bound IgE or specific IgE is quantified by a 125I-labelled anti-human IgE. The non-bound 125I-labelled anti-human IgE can be collected and used in new assay. By a 4-h incubation of the used paper discs with 1 M glycine-HCl buffer (pH 2.7) the IgE-labelled anti-IgE complex can largely be removed. The paper discs treated in this manner can be used in a new assay.[1]

References

  1. Reutilization of 125I-labelled anti-IgE antibody and paper discs in PRIST and RAST IgE determination. Bruynzeel, P.L., van den Bogaard, W., Berrens, L. Clin. Chim. Acta (1978) [Pubmed]
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