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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Determination of putrescine in brain tissue using gas chromatography-mass spectrometry.

We used gas chromatography-mass spectrometry to assay putrescine in minute regions of single rat brains. Acid extraction, partial purification on Amberlite CG 120, and derivatization with pentafluoropropionic anhydride preceded the gas chromatography-mass spectrometry. A moving-needle solventless system and a direct inlet system were also used to increase sensitivity. Putrescine was measured accurately at the picomole level; the mean concentration of this polyamine in five regions of rat brain found by this method was 2.7-3.8 times higher than reported by other researchers.[1]

References

  1. Determination of putrescine in brain tissue using gas chromatography-mass spectrometry. Noto, T., Hasegawa, T., Kamimura, H., Nakao, J., Hashimoto, H., Nakajima, T. Anal. Biochem. (1987) [Pubmed]
 
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