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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Determination of reducing and non-reducing carbohydrates in food products by liquid chromatography with post-column catalytic hydrolysis and derivatization. Comparison with refractive index detection.

Post-column catalytic hydrolysis combined with 4-aminobenzoic acid hydrazide derivatization is employed for the determination of both reducing and non-reducing carbohydrates in a variety of complex sample matrices such as dairy products, processed foods and tobaccos. Comparison with refractive index detection shows the post-column method to be superior from the standpoint of selectivity, sensitivity and simplicity of sample preparation. Limits of detection are in the low-nanogram range. Quantitative results are presented for the determination of sugars in potato extracts. The method is also applicable for the separation and detection of carbohydrate oligomers.[1]

References

  1. Determination of reducing and non-reducing carbohydrates in food products by liquid chromatography with post-column catalytic hydrolysis and derivatization. Comparison with refractive index detection. Femia, R.A., Weinberger, R. J. Chromatogr. (1987) [Pubmed]
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