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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Post-meiotic transcription of phosphoglycerate-kinase 2 in mouse testes.

We have used a human phosphoglycerate kinase-1 ( PGK-1) cDNA clone to study expression of PGK-2 during mouse spermatogenesis. Hybrid selection, in vitro translation with product identification by 2-D gel electrophoresis demonstrated that the PGK-1 cDNA clone hybridized to PGK-2 mRNA in mouse testes. Northern analyses of RNA purified from separated spermatogenic cells demonstrated a large increase in abundance of PGK-2 mRNA in post-meiotic cells. Thus, post-meiotic transcription of PGK-2 mRNA is demonstrable with cloned DNA probes.[1]

References

  1. Post-meiotic transcription of phosphoglycerate-kinase 2 in mouse testes. Erickson, R.P., Michelson, A.M., Rosenberg, M.P., Sánchez, E., Orkin, S.H. Biosci. Rep. (1985) [Pubmed]
 
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