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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Agarose gel electrophoresis of plasma lipoproteins using the Durrum cell.

1. A low cost method for lipoprotein electrophoresis in agarose gel is described. The basic procedure, described by Noble, has been modified for use with a Durrum cell which is commonly used for paper electrophoreis. 2. Up to 28 samples can be analyzed per Durrum cell in 4 hours. 3. A staining technique, which involves filtration of a suspension of Sudan Black B, allows for rapid staining and provides satisfactory reproducibility from run to run. 4. In a small number of patients a double pre-beta band is seen, and is different from sinking pre-beta and floating beta lipoproteins.[1]

References

  1. Agarose gel electrophoresis of plasma lipoproteins using the Durrum cell. Maguire, G.F., Breckenridge, W.C. Clin. Biochem. (1975) [Pubmed]
 
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