Disease relevance of CI 26150

• Sudan black B positivity in acute lymphoblastic leukemia [1].
• Therefore, Sudan Black B used alone to differentiate lymphoid from myeloid leukemia may be misleading [1].
• Negative stain for myeloid peroxidase and Sudan black B in acute promyelocytic leukemia (APL) cells: report of two patients with APL variant [2].
• A screening method based on differential staining of the wild type and exopolysaccharide-deficient mutants of Rhizobium (Sinorhizobium) meliloti by the lipophilic dye Sudan Black B is described [3].
• Sudan black B and peroxidase positivity in cultured lymphoid cells from patients with B-type chronic lymphocytic leukemia [4].

High impact information on CI 26150

• Positive myeloperoxidase and/or sudan black-B stainings and CD13 positivity in these cases were consistent with the presence of a myeloid involvement [5].
• Mitoses were identified as being of erythroid lineage by immunofluorescent staining for surface glycophorin A and as being of granulocytic lineage by cytoplasmic staining for Sudan black B [6].
• The phenotype of the leukemic cells was characterized by the presence of mature or early monocyte lineage antigens and intense Ia antigen expression detected by monoclonal antibodies, terminal deoxytransferase activity, and cytochemical features, including both Sudan black B and periodic acid-Schiff activity [7].
• Renal and hepatocyte storage material stained intensely with oil red O and moderately with periodic acid-Schiff/alcian blue and Sudan black B stains [8].
• A simple method was developed to identify large mononuclear (LMN) cells in human synovial fluid based on morphology and staining with Sudan black B [9].

Chemical compound and disease context of CI 26150

• The sensitivity of a cytochemical-staining combination of PAS positivity and myeloperoxidase, Sudan black B, and alpha-naphthyl butyrate esterase negativity in defining cases of lymphoblastic leukemia remained at 52%; however, the specificity of this combination for lymphoblastic leukemia was 100% (no false positives) [10].
• Sudan black B staining for frozen tissues of autopsied FAP patients and biospied dialysis related amyloidosis (DRA) patients revealed colocalization of lipids in the tissue sections where Congo red staining was positive, while no such positive staining was observed in paraffin embedded tissues [11].
• Picrosirius, Toluidine Blue and Sudan Black B methods were applied, respectively, for identification of collagen fibers in submandibular gland, sulfated glycosaminoglycans in cartilage (metachromasia) and myelin lipids in nervous ganglion [12].

Biological context of CI 26150

• Cell lineage was identified as erythroid by surface glycophorin A, granulocytic by Sudan black B and PM-81, and monocytic by lysozyme [13].
• Myeloid cytochemistry, i.e. peroxidase and Sudan black-B stain, was negative in all cases [14].
• A representative subline, LIa5 microM, was observed to have vesicles that reacted with Sudan black B stain, an indication of altered lipid metabolism [15].
• More than 100 mutants accumulating TAG at a significantly lower rate were obtained by chemical mutagenesis and identified by staining with Sudan Black B [16].
• Ten of these thirteen patients had myeloid markers such as Philadelphia chromosomes, peroxidase or Sudan Black B positive reactions, or Fc and C3 receptors [17].

Anatomical context of CI 26150

• There is accumulation of autofluorescent, periodic acid-Schiff-, and Sudan black B-positive granules that are resistant to lipid solvents in the cytoplasm of most nerve cells and. to a lesser degree, of many other cell types [18].
• Morphologically, after exposure to apoEko-VLDL/IDL, macrophages became strongly stained with Sudan black B [19].
• In leukemic myeloblasts and promyelocytes stained with basic blue 93, variable numbers of black-colored lysosomes could be detected and usually paralleled the reactions for myeloperoxidase and Sudan black B in the same cells [20].
• Mucicarminophilic histiocytes can be identified by the positive mucicarmine, colloidal iron, Gomori methenamine silver, Congo red, Sudan black B, Fontana-Masson argentaffin, and Victoria blue; but negative periodic acid-Schiff, alcian blue, and Giemsa stains [21].
• A(0) induced a characteristic series of changes, hallmarked by the formation of eosin- and Sudan Black-B-negative vacuoles [22].

Associations of CI 26150 with other chemical compounds

• HDL size phenotypes were based on nondenaturing gradient gel electrophoresis fractions after staining for esterified cholesterol with Sudan black B and for apoA-I with immunoblotting methods [23].
• The morphologic appearance of the leukemic cells coupled with the results of cytochemical staining reactions (alpha-naphthyl AS-D chloroacetate esterase, alpha-naphthyl butyrate esterase, Sudan black B, and periodic acid-Schiff reaction) identified this leukemia as myelomonocytic in origin [24].
• Neoplastic cells stained negatively for Sudan black B, myeloperoxidase, periodic acid-Schiff reagent, alpha-naphthyl acetate esterase, and acid phosphatase [25].
• Comparative studies showed that Sudan Black B is superior to Oil Red O for this purpose and that optimum staining is obtained using unfixed section or sections fixed in calcium-glutaraldehyde [26].
• Serum samples pre-stained with Sudan black B were electrophoresed using 4-30% polyacrylamide gradient gels, and the Stokes diameter of HDL particles was determined in 23 patients with genetic CETP deficiency, nine patients with hyperalphalipoproteinemia and seven subjects with normal HDL cholesterol concentrations [27].

Gene context of CI 26150

• Leukemic cells were characterized by scanty cytoplasm with fine azurophilic granules, and were highly positive for myeloperoxidase and sudan black-B [28].
• Anti-MPO was positive in all cases of AML (M1-M5) which were positive by Sudan Black B reaction in similar or higher percentage ratio for each case, although in some of them did not label with CD13/CD33 tested by IF and IPc techniques [29].
• Surgical specimens of atherosclerosis and chronic panniculitis containing MCLs as well as experimentally formed MCLs were examined, using paraffin sections, by Sudan black B (SBB) stain and by immunostaining for CD68, Bax protein and advanced glycation end products (AGEs) [30].
• Samples were separated by CITP combined with sudan black B staining [31].
• Expression of fibronectin and transforming growth factor-beta was greater in the Sudan black B-positive than in the Sudan black B-negative vessels, suggesting that extracellular matrix production was increased in vessels with lipid deposition [32].

Analytical, diagnostic and therapeutic context of CI 26150

• Morphologic and immunologic identification of mitotic cells can be done using routine hematologic stains, such as Giemsa or Sudan black B, and various antisera using immunofluorescence techniques [33].
• A high resolution electrophoretic method has been developed to separate plasma high density lipoprotein (HDL) particles by size using 4-30% polyacrylamide agarose (PAA) gradient gels, Sudan black B staining, and laser densitometry [34].
• 3. A staining technique, which involves filtration of a suspension of Sudan Black B, allows for rapid staining and provides satisfactory reproducibility from run to run [35].
• They were subjected to agarose gel electrophoresis, followed by staining with Sudan Black B, and their concentration was determined by optical densitometry [36].
• Leukocytes demonstrated cytoplasmic staining patterns very similar to mammalian leukocytes when stained with acid phosphatase, alpha-naphthyl butyrate esterase, beta-glucuronidase, alpha-naphthyl acetate esterase, Sudan Black B and anti-immunoglobulin specific immunohistochemistry [37].

References