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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Determination of bromhexine as its trifluoroacetyl derivative by gas-liquid chromatography with electron-capture detection.

A specific gas-liquid chromatographic method with electron-capture detection is described for the determination of bromhexine at the nanogram level. Structurally analogous internal standards were synthesized and their suitability was investigated, based on their chromatographic properties on different stationary liquid phases. The electron-capture activity of the compounds is increased by trifluoroacetylation. Reaction conditions for this derivatization were studied. Calibration graphs in the range 0-57 ng (in a total of 100 microliters of reaction mixture) showed good linearity.[1]


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