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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Expression of a biologically active fragment of human IgE epsilon chain in Escherichia coli.

cDNA corresponding to human IgE heavy (epsilon) chain mRNA was cloned from human IgE-secreting myeloma U266 cells. Partial nucleotide sequence analysis demonstrated that the cloned cDNA contained the coding region for about two-thirds of the CH2 and all of the CH3 and CH4 domains as well as the 3'-untranslated region. This epsilon cDNA was inserted into expression vector pUC7 and expression of an epsilon-chain fragment in Escherichia coli was demonstrated by protein blot analysis using 125I-labeled goat anti-human IgE as probe. The expression product was purified on a column of goat anti-human IgE-conjugated Sepharose 4B and the polypeptide was found to retain binding activity to human basophils.[1]

References

  1. Expression of a biologically active fragment of human IgE epsilon chain in Escherichia coli. Liu, F.T., Albrandt, K.A., Bry, C.G., Ishizaka, T. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
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