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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

An optimized assay of alanine aminopeptidase activity in urine.

Elaboration of an optimized kinetic test for urinary alanine aminopeptidase at 37 degrees C is described. As final concentrations, 50 mmol/L tris(hydroxymethyl)aminomethane x HCl buffer, pH 7.8, and 2 mmol/L alanine-4-nitroanilide are proposed. Five- to sixfold activitiy is obtained as compared with a generally used test procedure. Coefficients of variation for the method (within-run and between-day precision) ranged from 1.56 to 3.45%. Men excreted more alanine aminopeptidase than did women if urinary volume or urinary timed volume was used as basis of reference. We saw no sex-related differences in excretion of alanine aminopeptidase when enzyme excretion was related to urinary creatinine.[1]


  1. An optimized assay of alanine aminopeptidase activity in urine. Jung, K., Scholz, D. Clin. Chem. (1980) [Pubmed]
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