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Berbel, P.J. et al.

Handling Golgi-impregnated tissue for light microscopy.

The use of cyanocrylic glue to fix pieces of Golgi-stained nervous tissue on a paraffin blank is proposed for obtaining thick sections of unembedded tissue with a sliding microtome. This procedure makes correct orientation of the tissue easy during sectioning and makes it possible to obtain tissue sections quickly. The sections are flat-mounted using epoxy resin, resulting in permanent preparations with excellent optical properties and enabling further thin-sectioning for light and electron microscopic studies.[1]

References

Handling Golgi-impregnated tissue for light microscopy. Berbel, P.J., Fairén, A. Neurosci. Lett. (1983) [Pubmed]

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