Disease relevance of Microtomy

• The osmium fixation is, however, not strong enough to allow for a preparation of the vesicles for thin sectioning (dehydration, embedding in epoxide) [1].
• The ultrastructure of Streptococcus mutans, serotypes a-e, S. sanguis, S. mitis, and S. salivarius HHT, were examined by the techniques of thin-sectioning and freeze-etching [2].

High impact information on Microtomy

• Ultrastructural changes of the luminal plasma membrane were studied with thin sectioning and freeze-fracturing techniques [3].
• Ethanol treatment of the block face of the sample after microtomy elutes non-cross-linked polymer chains and makes the smallest details of the embedded biomaterial amenable to detection [4].
• For the TEM examination, the sections were partly decalcified by an aqueous EDTA solution to facilitate ultramicrotomy and to disclose the ultrastructure of the interdiffusion zone [5].
• Thin-sectioning studies confirmed the image features seen by other techniques, but the addition of tannic acid in the fixation procedure was required to visualize the structure [6].
• Cryo-sectioning and chemical-fixing ultramicrotomy techniques for imaging rubber latex particle morphology [7].

Chemical compound and disease context of Microtomy

• Standard aldehyde and osmium fixation, dehydration and epoxy embedding are compatible with this as is semi-thin sectioning for light microscopy or thin sectioning for electron microscopy [8].

Anatomical context of Microtomy

• The two synaptic types are distinguished on the basis of (a) the shape and position of the presynaptic density seen by serial thin sectioning and (b) the arrangement and location of vesicle attachment sites (VAS) on the presynaptic membrane obtained from replicas of aldehyde-fixed, freeze-fractured neuropile [9].
• "Cholinergic" synapses of the bullfrog sympathetic ganglion cells were investigated with thin sectioning, complementary freeze-fracturing, and deep-etching methods after glutaraldehyde fixation [10].
• Freeze-fracture and thin-sectioning have been used to examine the stria vascularis of albino guinea pigs chronically treated with gentamicin [11].
• After incubation, spermatozoa were fixed in glutaraldehyde, stained with osmium tetroxide and ruthenium red and embedded in Spurr(R)-resin followed by ultramicrotomy [12].

Associations of Microtomy with chemical compounds

• The sections are flat-mounted using epoxy resin, resulting in permanent preparations with excellent optical properties and enabling further thin-sectioning for light and electron microscopic studies [13].
• Ultramicrotomy of industrial alumina [14].
• Uranium-contaminated soils: ultramicrotomy and electron beam analysis [15].
• In this correlative study, also employing thin sectioning, freeze-fracture and deep-etching techniques in conjunction with the polyene antibiotic filipin, it could be shown that LDL preferentially binds to certain microdomains of the plasma membrane, the so-called coated pits [16].
• Bony sites containing the implant were prepared for ultramicrotomy and transmission electron microscopy using an anhydrous embedding procedure: fixation in ethylene glycol and embedment in Spurr's resin [17].

Gene context of Microtomy

• The AFM images clearly demonstrated the enhanced potential of diamond-knife microtomy for nondestructively producing clean cross-sections through interfaces that allow the interfacial ultra-structure to be imaged by AFM with a resolution equaling that of TEM [18].
• Using dual immunostaining and thin sectioning techniques, the ventrolateral SCN of light-exposed rats was examined for evidence of individual neurons coexpressing nuclear immunostaining for c-fos proteins (Fos) with cytoplasmic immunoreactivity for GRP or VIP [19].

Analytical, diagnostic and therapeutic context of Microtomy

• Negative staining, positive staining for thin sectioning, and freeze-fracture electron microscopy were used to characterize these synthetic vesicles and to evaluate the liposome-mineral interactions resulting from apatite formation [20].
• Metacestodes of E. granulosus (19 mo old), T. crassiceps (28 days old), and T. taeniaeformis (34 days old) from gerbils, mice and rats, respectively, were fixed for 2 hr in 3% glutaraldehyde and then prepared for freeze-etching and thin sectioning by standard techniques [21].
• The process is termed 'hot knife microtomy'. In three different laboratories, primary aldehyde fixation by perfusion and hot knife microtomy have given uniformly excellent data from normal, diseased, and virus-infected brain tissues [22].

References