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Escherichia coli glutaminyl-tRNA synthetase. I. Isolation and DNA sequence of the glnS gene.

We have isolated a lambda-transducing phage carrying the gene (glnS) for Escherichia coli glutaminyl-tRNA synthetase. The location of the glnS gene within the 13.5-kilobase E. coli DNA transducing fragment was determined by genetic means. The glnS gene was recloned into plasmid pBR322 and its nucleotide sequence was established. The DNA sequence translates to a protein of 550 amino acids.[1]

References

  1. Escherichia coli glutaminyl-tRNA synthetase. I. Isolation and DNA sequence of the glnS gene. Yamao, F., Inokuchi, H., Cheung, A., Ozeki, H., Söll, D. J. Biol. Chem. (1982) [Pubmed]
 
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