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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Q beta replicase containing a Bacillus stearothermophilus elongation factor.

We purified Q beta replicase containing EF-Ts from Bacillus stearothermophilus in place of the homologous polypeptide from Escherichia coli. The hybrid enzyme was fully active in the transcription of a variety of templates. It was found to be qualitatively similar to native Q beta replicase with respect to a variety of parameters which measure the efficiency of initiation of RNA synthesis. The results demonstrated that Q beta replicase can tolerate substantial alterations in the EF-Tu X Ts component of the enzyme. These alterations resulted in only minor perturbations of catalytic properties.[1]


  1. Q beta replicase containing a Bacillus stearothermophilus elongation factor. Stringfellow, L., Blumenthal, T. J. Bacteriol. (1983) [Pubmed]
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