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Gorden, P. et al.

Intracellular translocation of iodine-125-labeled insulin: direct demonstration in isolated hepatocytes.

Insulin labeled with iodine-125 binds to receptors on isolated rat hepatocytes. At low temperatures initial binding is restricted to the plasma membrane as detected by direct quantitative autoradiographic analysis with the electron microscope. With increasing time and temperature of incubation there is a systematic and progressive translocation of autoradiographic grains to a highly limited area of the cell periphery representing no more than 15% of the radius of the cell.[1]

References

Intracellular translocation of iodine-125-labeled insulin: direct demonstration in isolated hepatocytes. Gorden, P., Carpentier, J.L., Freychet, P., LeCam, A., Orci, L. Science (1978) [Pubmed]

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