The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Determination of urinary thiamine by high-pressure liquid chromatography utilizing the thiochrome fluorscent method.

A sensitive, reproducible, and specific method for the determination of urinary thiamine has been established. Unique to the use of high-pressure liquid chromatography (HPLC) to separate the fluorescent thiamine derivative from interfering fluorescent compounds. Urine samples were passed through a Decalso catoin-exchange column, washed with 0.5 M KCl to remove some interfering compounds, and eluted with 3.4 M KCl. The eluted thiamine was converted to the fluorescent derivative, thiochrome, by reaction with alkaline potassium ferricyanide. The reaction mixture was extracted with isobutanol and subjected to HPLC monitored by a fluorescent detector. Within-day and day-to-day coefficients of variation proved to be 2.5% and 1.2% respectively. Recovery of added thiamine (range 0.04 to 2.0 microgram/ml) averaged 99.9 +/- 5.3%. The sensitivity of this method was 0.03 microgram/ml.[1]

References

  1. Determination of urinary thiamine by high-pressure liquid chromatography utilizing the thiochrome fluorscent method. Roser, R.L., Andrist, A.H., Harrington, W.H., Naito, H.K., Lonsdale, D. J. Chromatogr. (1978) [Pubmed]
 
WikiGenes - Universities