Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Challen, A. et al.

Quantitation of platelet mass during aggregation in the electronic (Wellcome) whole blood aggregometer.

A new electronic aggregometer has been described which measures platelet aggregation by monitoring the changes in electrical impedance that occur when a platelet aggregate forms between two electrodes (Cardinal and Flower, 1980). To validate the instrument, the protein content of the platelet aggregate was estimated by the Lowry method (Lowry et al., 1951). Aggregating agents used were ADP (doses (mu mol), 1.0, 0.1, 0.01, 0.005, 0.0025 and 0.001), Thrombin (doses (units) 0.25, 0.20, 0.15, 0.10 and 0.05) and Collagen (doses (micrograms) 20.0, 15.0, 10.0, 5.0, 1.0 and 0.5). The quantity of protein was correlated with the dose of aggregating agent and with 6 characteristics of the aggregation curve. Linear correlation coefficients of the former were 0.92 (Log dose ADP), 0.91 (Thrombin dose) and 0.86 (Collagen dose). Correlation coefficients (Electrode Protein against curve characteristics), were greater than 0.87 for all aggregating agents and the extent of aggregation (ohms), percent of maximum aggregation, percent of maximum aggregation at 6 minutes, and maximum rate of aggregation (ohms/s). Linear correlation coefficients for the curve characteristics, time to maximum aggregation(s) and time to 50% of maximum aggregation(s), for ADP and Collagen were greater than 0.70. However, correlation coefficients of the above two characteristics to Thrombin were 0.32. We have concluded that the maximum extent of aggregation is the best method of assessing platelet aggregation with the electronic aggregometer.[1]

References

Quantitation of platelet mass during aggregation in the electronic (Wellcome) whole blood aggregometer. Challen, A., Branch, W.J., Cummings, J.H. Journal of pharmacological methods. (1982) [Pubmed]

Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.