Temperature-sensitive glutamate dehydrogenase mutants of Salmonella typhimurium.
Mutants of Salmonella typhimurium defective in glutamate dehydrogenase activity were isolated in parent strains lacking glutamate synthase activity by localizcd mutagenesis or by a general mutagenesis combined with a cycloserine enrichment for glutamate auxotrophs. Two mutants with temperature-sensitive phenotypes had glutamate dehydrogenase activities that were more thermolabile than that of an isogenic control strain. Eight other mutants had less than 10% of the wild-type glutamate dehydrogenase activity. All the mutations were cotransducible with a Tn10 element (zed-2:Tn10) located at approximately 23 U on the S. typhimurium linkage map. These data strongly indicate that this region contains the structural gene (gdhA) for glutamate dehydrogenase.[1]References
- Temperature-sensitive glutamate dehydrogenase mutants of Salmonella typhimurium. Dendinger, S.M., Brenchley, J.E. J. Bacteriol. (1980) [Pubmed]
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