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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
MeSH Review

Mutagenesis

 
 
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Disease relevance of Mutagenesis

  • SOD-null mutants have been prepared to reveal the biological effects of O2-. SodA, sodB E. coli exhibit dioxygen-dependent auxotrophies and enhanced mutagenesis, reflecting O2(-)-sensitive biosynthetic pathways and DNA damage [1].
  • Saturation mutagenesis and a complement fixation selection have yielded CD4 point mutants with impaired antibody and human immunodeficiency virus binding [2].
  • The halogenated thymidine (dT) analogue, 5-bromodeoxy-uridine (BUdR), has a variety of effects on mammalian cells, including toxicity, suppression of differentiation, and mutagenesis [3].
  • We have proposed that recA protein is also activated to attack repressors in vivo when it interacts with single-stranded DNA; DNA-damaging treatments such as UV-ray irradiation would thereby invoke the recA-dependent functions of recombination, repair, mutagenesis and prophage induction [4].
  • Second site suppressors of the dunce (dnc) female sterility phenotype were isolated with the use of transposon mutagenesis [5].
 

Psychiatry related information on Mutagenesis

 

High impact information on Mutagenesis

  • Secondly, advances in molecular modeling and mutagenesis of this ligand-receptor pair have helped to identify the critical receptor/ligand contacts in the gp39/CD40 complex [7].
  • Included is a discussion of available sources and assays for TS, a description of the enzyme's chemical mechanism and crystal structure, and a summary of data obtained from mutagenesis experiments [8].
  • Identification and validation of PDGF transcriptional targets by microarray-coupled gene-trap mutagenesis [9].
  • Mechanisms of autoinhibition and STI-571/imatinib resistance revealed by mutagenesis of BCR-ABL [10].
  • We provide support for this approach and report the identification of mutations in the gene (Gjb2) encoding connexin 26, using archives established from the UK ENU mutagenesis program [11].
 

Chemical compound and disease context of Mutagenesis

 

Biological context of Mutagenesis

 

Anatomical context of Mutagenesis

 

Associations of Mutagenesis with chemical compounds

  • Using alanine-scanning mutagenesis, we have found that microtubule-interacting kinesin residues are located in three loops that cluster in a patch on the motor surface [27].
  • Previous work with bacteria has shown that a gene is maximally sensitive to mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine (NG) at the time it is being replicated [28].
  • Yeast cells growing exponentially were more sensitive to mutagenesis by NG than cells in which DNA synthesis had been inhibited [28].
  • To test this hypothesis, we modified the cytoplasmic serine residues or introduced a deletion of 36 amino acids by in vitro mutagenesis of a cDNA expression vector for human TR [29].
  • Deletion mutagenesis demonstrated that CRT inhibition was mediated by the high affinity-low capacity Ca(2+)-binding domain, which contributes little to Ca2+ storage [30].
 

Gene context of Mutagenesis

  • However, genetic studies of C. elegans and Drosophila, as well as the targeted mutagenesis of the murine Araf gene, have failed to support such a role [31].
  • Here we use targeted mutagenesis to explore the role of Zfpm2 in normal cardiac development [32].
  • A combination of chromosomal walking and transposon-mediated mutagenesis was used to clone the norpA gene [33].
  • We assessed the role of KH domains in RNA binding by mutagenesis of KH domains in hnRNP K and FMR1 [34].
  • We identified a new allele of hairy in a mutagenesis screen and showed that hairy mutations cause branching and bulging of the normally unbranched salivary tube, in part through prolonged expression of huckebein (hkb) [35].
 

Analytical, diagnostic and therapeutic context of Mutagenesis

  • We have used mutagenesis and in vitro selection strategies to show that RNA cleavage and ligation has an absolute requirement for guanosine immediately 3' to the cleavage-ligation site [36].
  • Rescue of this strain by mutant CDC28 was dependent upon the mutant cln2-KAEA, but additional mutagenesis and DNA shuffling yielded multiply mutant CDC28-BYC alleles (bypass of CLNs) that could support highly efficient cell cycle initiation in the complete absence of CLN genes [37].
  • We created temperature-sensitive SPC110 mutations by combining PCR mutagenesis with a plasmid shuffle strategy [38].
  • Here we used peptide mapping, mass spectroscopy analysis, and mutagenesis to identify the nature of the link between RanGAP1 and SUMO-1 [39].
  • Kinetic and sequence analysis coupled with mutagenesis, structural, and modeling studies reveal that the defining event in the evolution of these catalysts was a somatic mutation that placed a lysine residue in a deep, yet otherwise unrefined, hydrophobic pocket [40].

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  23. Genetic evidence for selective transport of opsin and arrestin by kinesin-II in mammalian photoreceptors. Marszalek, J.R., Liu, X., Roberts, E.A., Chui, D., Marth, J.D., Williams, D.S., Goldstein, L.S. Cell (2000) [Pubmed]
  24. Tentoxin sensitivity of chloroplasts determined by codon 83 of beta subunit of proton-ATPase. Avni, A., Anderson, J.D., Holland, N., Rochaix, J.D., Gromet-Elhanan, Z., Edelman, M. Science (1992) [Pubmed]
  25. E-MAP-115, encoding a microtubule-associated protein, is a retinoic acid-inducible gene required for spermatogenesis. Komada, M., McLean, D.J., Griswold, M.D., Russell, L.D., Soriano, P. Genes Dev. (2000) [Pubmed]
  26. Activation of Bruton's tyrosine kinase (BTK) by a point mutation in its pleckstrin homology (PH) domain. Li, T., Tsukada, S., Satterthwaite, A., Havlik, M.H., Park, H., Takatsu, K., Witte, O.N. Immunity (1995) [Pubmed]
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