The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review


Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Mutagenesis

  • SOD-null mutants have been prepared to reveal the biological effects of O2-. SodA, sodB E. coli exhibit dioxygen-dependent auxotrophies and enhanced mutagenesis, reflecting O2(-)-sensitive biosynthetic pathways and DNA damage [1].
  • Saturation mutagenesis and a complement fixation selection have yielded CD4 point mutants with impaired antibody and human immunodeficiency virus binding [2].
  • The halogenated thymidine (dT) analogue, 5-bromodeoxy-uridine (BUdR), has a variety of effects on mammalian cells, including toxicity, suppression of differentiation, and mutagenesis [3].
  • We have proposed that recA protein is also activated to attack repressors in vivo when it interacts with single-stranded DNA; DNA-damaging treatments such as UV-ray irradiation would thereby invoke the recA-dependent functions of recombination, repair, mutagenesis and prophage induction [4].
  • Second site suppressors of the dunce (dnc) female sterility phenotype were isolated with the use of transposon mutagenesis [5].

Psychiatry related information on Mutagenesis


High impact information on Mutagenesis

  • Secondly, advances in molecular modeling and mutagenesis of this ligand-receptor pair have helped to identify the critical receptor/ligand contacts in the gp39/CD40 complex [7].
  • Included is a discussion of available sources and assays for TS, a description of the enzyme's chemical mechanism and crystal structure, and a summary of data obtained from mutagenesis experiments [8].
  • Identification and validation of PDGF transcriptional targets by microarray-coupled gene-trap mutagenesis [9].
  • Mechanisms of autoinhibition and STI-571/imatinib resistance revealed by mutagenesis of BCR-ABL [10].
  • We provide support for this approach and report the identification of mutations in the gene (Gjb2) encoding connexin 26, using archives established from the UK ENU mutagenesis program [11].

Chemical compound and disease context of Mutagenesis


Biological context of Mutagenesis


Anatomical context of Mutagenesis


Associations of Mutagenesis with chemical compounds

  • Using alanine-scanning mutagenesis, we have found that microtubule-interacting kinesin residues are located in three loops that cluster in a patch on the motor surface [27].
  • Previous work with bacteria has shown that a gene is maximally sensitive to mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine (NG) at the time it is being replicated [28].
  • Yeast cells growing exponentially were more sensitive to mutagenesis by NG than cells in which DNA synthesis had been inhibited [28].
  • To test this hypothesis, we modified the cytoplasmic serine residues or introduced a deletion of 36 amino acids by in vitro mutagenesis of a cDNA expression vector for human TR [29].
  • Deletion mutagenesis demonstrated that CRT inhibition was mediated by the high affinity-low capacity Ca(2+)-binding domain, which contributes little to Ca2+ storage [30].

Gene context of Mutagenesis

  • However, genetic studies of C. elegans and Drosophila, as well as the targeted mutagenesis of the murine Araf gene, have failed to support such a role [31].
  • Here we use targeted mutagenesis to explore the role of Zfpm2 in normal cardiac development [32].
  • A combination of chromosomal walking and transposon-mediated mutagenesis was used to clone the norpA gene [33].
  • We assessed the role of KH domains in RNA binding by mutagenesis of KH domains in hnRNP K and FMR1 [34].
  • We identified a new allele of hairy in a mutagenesis screen and showed that hairy mutations cause branching and bulging of the normally unbranched salivary tube, in part through prolonged expression of huckebein (hkb) [35].

Analytical, diagnostic and therapeutic context of Mutagenesis

  • We have used mutagenesis and in vitro selection strategies to show that RNA cleavage and ligation has an absolute requirement for guanosine immediately 3' to the cleavage-ligation site [36].
  • Rescue of this strain by mutant CDC28 was dependent upon the mutant cln2-KAEA, but additional mutagenesis and DNA shuffling yielded multiply mutant CDC28-BYC alleles (bypass of CLNs) that could support highly efficient cell cycle initiation in the complete absence of CLN genes [37].
  • We created temperature-sensitive SPC110 mutations by combining PCR mutagenesis with a plasmid shuffle strategy [38].
  • Here we used peptide mapping, mass spectroscopy analysis, and mutagenesis to identify the nature of the link between RanGAP1 and SUMO-1 [39].
  • Kinetic and sequence analysis coupled with mutagenesis, structural, and modeling studies reveal that the defining event in the evolution of these catalysts was a somatic mutation that placed a lysine residue in a deep, yet otherwise unrefined, hydrophobic pocket [40].


  1. Superoxide radical and superoxide dismutases. Fridovich, I. Annu. Rev. Biochem. (1995) [Pubmed]
  2. Genetic analysis of monoclonal antibody and HIV binding sites on the human lymphocyte antigen CD4. Peterson, A., Seed, B. Cell (1988) [Pubmed]
  3. Induction of sister chromatid exchanges by BUdR is largely independent of the BUdR content of DNA. Davidson, R.L., Kaufman, E.R., Dougherty, C.P., Ouellette, A.M., DiFolco, C.M., Latt, S.A. Nature (1980) [Pubmed]
  4. Two mutations that alter the regulatory activity of E. coli recA protein. Roberts, J.W., Roberts, C.W. Nature (1981) [Pubmed]
  5. A neuropeptide gene defined by the Drosophila memory mutant amnesiac. Feany, M.B., Quinn, W.G. Science (1995) [Pubmed]
  6. Identification of linotte, a new gene affecting learning and memory in Drosophila melanogaster. Dura, J.M., Preat, T., Tully, T. J. Neurogenet. (1993) [Pubmed]
  7. Immune regulation by CD40 and its ligand GP39. Foy, T.M., Aruffo, A., Bajorath, J., Buhlmann, J.E., Noelle, R.J. Annu. Rev. Immunol. (1996) [Pubmed]
  8. The catalytic mechanism and structure of thymidylate synthase. Carreras, C.W., Santi, D.V. Annu. Rev. Biochem. (1995) [Pubmed]
  9. Identification and validation of PDGF transcriptional targets by microarray-coupled gene-trap mutagenesis. Chen, W.V., Delrow, J., Corrin, P.D., Frazier, J.P., Soriano, P. Nat. Genet. (2004) [Pubmed]
  10. Mechanisms of autoinhibition and STI-571/imatinib resistance revealed by mutagenesis of BCR-ABL. Azam, M., Latek, R.R., Daley, G.Q. Cell (2003) [Pubmed]
  11. A gene-driven approach to the identification of ENU mutants in the mouse. Coghill, E.L., Hugill, A., Parkinson, N., Davison, C., Glenister, P., Clements, S., Hunter, J., Cox, R.D., Brown, S.D. Nat. Genet. (2002) [Pubmed]
  12. Transcriptional mutagenesis induced by uracil and 8-oxoguanine in Escherichia coli. Brégeon, D., Doddridge, Z.A., You, H.J., Weiss, B., Doetsch, P.W. Mol. Cell (2003) [Pubmed]
  13. Molecular analysis of a mutant defective in photosynthetic oxygen evolution and isolation of a complementing clone by a novel screening procedure. Dzelzkalns, V.A., Bogorad, L. EMBO J. (1988) [Pubmed]
  14. The pathway for perception and transduction of low-temperature signals in Synechocystis. Suzuki, I., Los, D.A., Kanesaki, Y., Mikami, K., Murata, N. EMBO J. (2000) [Pubmed]
  15. Targeted mutagenesis of the psbE and psbF genes blocks photosynthetic electron transport: evidence for a functional role of cytochrome b559 in photosystem II. Pakrasi, H.B., Williams, J.G., Arntzen, C.J. EMBO J. (1988) [Pubmed]
  16. Near ultraviolet DNA damage induces the SOS responses in Escherichia coli. Caldeira de Araujo, A., Favre, A. EMBO J. (1986) [Pubmed]
  17. A small chloroplast RNA may be required for trans-splicing in Chlamydomonas reinhardtii. Goldschmidt-Clermont, M., Choquet, Y., Girard-Bascou, J., Michel, F., Schirmer-Rahire, M., Rochaix, J.D. Cell (1991) [Pubmed]
  18. Genetic and biochemical characterization of mutants at an RNA polymerase II locus in D. melanogaster. Greenleaf, A.L., Weeks, J.R., Voelker, R.A., Ohnishi, S., Dickson, B. Cell (1980) [Pubmed]
  19. A single glutamic acid residue plays a key role in the transcriptional activation function of lambda repressor. Bushman, F.D., Shang, C., Ptashne, M. Cell (1989) [Pubmed]
  20. Phosphorylation of the MAP kinase ERK2 promotes its homodimerization and nuclear translocation. Khokhlatchev, A.V., Canagarajah, B., Wilsbacher, J., Robinson, M., Atkinson, M., Goldsmith, E., Cobb, M.H. Cell (1998) [Pubmed]
  21. Targeted mutagenesis of Tsix leads to nonrandom X inactivation. Lee, J.T., Lu, N. Cell (1999) [Pubmed]
  22. E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo. Porse, B.T., Pedersen TA, n.u.l.l., Xu, X., Lindberg, B., Wewer, U.M., Friis-Hansen, L., Nerlov, C. Cell (2001) [Pubmed]
  23. Genetic evidence for selective transport of opsin and arrestin by kinesin-II in mammalian photoreceptors. Marszalek, J.R., Liu, X., Roberts, E.A., Chui, D., Marth, J.D., Williams, D.S., Goldstein, L.S. Cell (2000) [Pubmed]
  24. Tentoxin sensitivity of chloroplasts determined by codon 83 of beta subunit of proton-ATPase. Avni, A., Anderson, J.D., Holland, N., Rochaix, J.D., Gromet-Elhanan, Z., Edelman, M. Science (1992) [Pubmed]
  25. E-MAP-115, encoding a microtubule-associated protein, is a retinoic acid-inducible gene required for spermatogenesis. Komada, M., McLean, D.J., Griswold, M.D., Russell, L.D., Soriano, P. Genes Dev. (2000) [Pubmed]
  26. Activation of Bruton's tyrosine kinase (BTK) by a point mutation in its pleckstrin homology (PH) domain. Li, T., Tsukada, S., Satterthwaite, A., Havlik, M.H., Park, H., Takatsu, K., Witte, O.N. Immunity (1995) [Pubmed]
  27. Microtubule interaction site of the kinesin motor. Woehlke, G., Ruby, A.K., Hart, C.L., Ly, B., Hom-Booher, N., Vale, R.D. Cell (1997) [Pubmed]
  28. Temporal order in yeast chromosome replication. Burke, W., Fangman, W.L. Cell (1975) [Pubmed]
  29. Endocytosis of the transferrin receptor requires the cytoplasmic domain but not its phosphorylation site. Rothenberger, S., Iacopetta, B.J., Kühn, L.C. Cell (1987) [Pubmed]
  30. Calreticulin inhibits repetitive intracellular Ca2+ waves. Camacho, P., Lechleiter, J.D. Cell (1995) [Pubmed]
  31. Endothelial apoptosis in Braf-deficient mice. Wojnowski, L., Zimmer, A.M., Beck, T.W., Hahn, H., Bernal, R., Rapp, U.R., Zimmer, A. Nat. Genet. (1997) [Pubmed]
  32. A syndrome of tricuspid atresia in mice with a targeted mutation of the gene encoding Fog-2. Svensson, E.C., Huggins, G.S., Lin, H., Clendenin, C., Jiang, F., Tufts, R., Dardik, F.B., Leiden, J.M. Nat. Genet. (2000) [Pubmed]
  33. Isolation of a putative phospholipase C gene of Drosophila, norpA, and its role in phototransduction. Bloomquist, B.T., Shortridge, R.D., Schneuwly, S., Perdew, M., Montell, C., Steller, H., Rubin, G., Pak, W.L. Cell (1988) [Pubmed]
  34. Essential role for KH domains in RNA binding: impaired RNA binding by a mutation in the KH domain of FMR1 that causes fragile X syndrome. Siomi, H., Choi, M., Siomi, M.C., Nussbaum, R.L., Dreyfuss, G. Cell (1994) [Pubmed]
  35. Epithelial tube morphology is determined by the polarized growth and delivery of apical membrane. Myat, M.M., Andrew, D.J. Cell (2002) [Pubmed]
  36. Novel guanosine requirement for catalysis by the hairpin ribozyme. Chowrira, B.M., Berzal-Herranz, A., Burke, J.M. Nature (1991) [Pubmed]
  37. Directed evolution to bypass cyclin requirements for the Cdc28p cyclin-dependent kinase. Levine, K., Kiang, L., Jacobson, M.D., Fisher, R.P., Cross, F.R. Mol. Cell (1999) [Pubmed]
  38. Role of calmodulin and Spc110p interaction in the proper assembly of spindle pole body compenents. Sundberg, H.A., Goetsch, L., Byers, B., Davis, T.N. J. Cell Biol. (1996) [Pubmed]
  39. Molecular characterization of the SUMO-1 modification of RanGAP1 and its role in nuclear envelope association. Mahajan, R., Gerace, L., Melchior, F. J. Cell Biol. (1998) [Pubmed]
  40. Using antibody catalysis to study the outcome of multiple evolutionary trials of a chemical task. Karlstrom, A., Zhong, G., Rader, C., Larsen, N.A., Heine, A., Fuller, R., List, B., Tanaka, F., Wilson, I.A., Barbas, C.F., Lerner, R.A. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
WikiGenes - Universities