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Dux, L. et al.

The value of simple lipid stains for typing skeletal muscle fibres.

Skeletal muscle fibre types can be distinguished rapidly with simple lipid stains. Comparative studies showed that Sudan Black B is superior to Oil Red O for this purpose and that optimum staining is obtained using unfixed section or sections fixed in calcium-glutaraldehyde. Factors that possibly influence the staining reaction, such as freeze-thawing, are considered. The stained lipids were identified by thin layer chromatography.[1]

References

The value of simple lipid stains for typing skeletal muscle fibres. Dux, L., Mészáros, M.G., Rohán, J., Gajdos, L., Jakab, G., Guba, F. Histochem. J. (1981) [Pubmed]

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