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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

How pleural mesothelial cells react in vitro with chrysotile fibres.

A culture system of rat pleural mesothelial cells has been developed for short-term experiments and long-term maintenance. In short-term experiments, endocytosis of chrysotile fibres (100 microgram/ml) has been demonstrated by electron microscopy of the pleura in situ. Structural modifications, mainly cytoplasmic vacuolization, were observed when the cells were cultured with sublethal doses of chrysotile (0.5, 1, 2 and 10 micrograms/ml). Untreated cells had a mean population doubling time of 37 hours. When the cells were treated with 2 or 10 micrograms/ml, the mean population doubling time was slightly increased.[1]

References

  1. How pleural mesothelial cells react in vitro with chrysotile fibres. Kaplan, H., Jaurand, M.C., Pinchon, M.C., Bernaudin, J.F., Bignon, J., Adnet, J.J. IARC Sci. Publ. (1980) [Pubmed]
 
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