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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Partial purification and separation of H-2 antigens by free-flow preparative electrophoresis.

The membrane fraction of murine spleen cells was solubilized by autolysis in 0.25% potassium cholate and 3M potassium chloride, and the solubilized membrane components were fractionated by ultrafiltration and preparative free-flow electrophoresis. This procedure yielded partially purified fractions with enriched specific antigenic activities. The H-2Ka and H-2Da antigens were found to possess distinctly different electrophoretic mobilities.[1]

References

  1. Partial purification and separation of H-2 antigens by free-flow preparative electrophoresis. Koubek, K., Kristofová, H., Rieger, M., Hilgert, I. Folia Biol. (Praha) (1980) [Pubmed]
 
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