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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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7 alpha-Dehydroxylation of cholic acid by cell extracts of Eubacterium species V.P.I. 12708.

NADH:flavin oxidoreductase and 7 alpha-dehydroxylase were induced 5- and 90-fold, respectively, by cholic acid in cultures of Eubacterium species V.P.I. 12708. Assays of 7 alpha-dehydroxylase activity in the presence of various cofactors revealed that optimal activity was obtained in the presence of NAD+ plus FADH2. The pH optima of 7 alpha-dehydroxylase activity in whole cells and cell extracts were 7. 0. The similar induction pattern of these two enzymes and the apparent cofactor requirements for 7 alpha-dehydroxylation suggest a relationship between 7 alpha-dehydroxylase and NADH:flavin oxidoreductase.[1]

References

  1. 7 alpha-Dehydroxylation of cholic acid by cell extracts of Eubacterium species V.P.I. 12708. Hylemon, P.B., Cacciapuoti, A.F., White, B.A., Whitehead, T.R., Fricke, R.J. Am. J. Clin. Nutr. (1980) [Pubmed]
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