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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Fv fragment-mediated crystallization of the membrane protein bacterial cytochrome c oxidase.

Crystallization of membrane proteins, a prerequisite for their X-ray crystallographic analysis, remains difficult. Here, we demonstrate that the crystallization of the cytochrome c oxidase from Paracoccus denitrificans can be mediated by co-crystallization with an antibody Fv fragment. The crystals obtained contain all four subunits of this membrane protein complex and the Fv fragment. The approach of co-crystallizing membrane proteins with antibody fragments should be useful in obtaining well-ordered crystals of membrane proteins in general.[1]

References

  1. Fv fragment-mediated crystallization of the membrane protein bacterial cytochrome c oxidase. Ostermeier, C., Iwata, S., Ludwig, B., Michel, H. Nat. Struct. Biol. (1995) [Pubmed]
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