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Biotinylated L-selectin ligand analogs as cytochemical probes in cytospin preparations.

Amino-derivatives of L-selectin ligand analogs: phosphonoester core polysaccharide (PPME) and fucoidin were biotinylated with the use of biotinyl-N-succinimide ester, and these biotinylated analogs b-PPME and b-fucoidin were demonstrated as useful tools to investigate the functional activity of L-selectins in cytospin preparations obtained from healthy human donors and from patients with chronic lymphocytic leukemia (CLL). The avidin/biotin system adds a new alternative to the application of the L-selectin ligand analogs (PPME, fucoidin) which have been formerly used as fluoresceinated, solid phase or immobilized probes.[1]

References

  1. Biotinylated L-selectin ligand analogs as cytochemical probes in cytospin preparations. Vass, J.A., Csanaky, G. Folia Histochem. Cytobiol. (1995) [Pubmed]
 
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