Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Egaas, E. et al.

The separation and identification of glutathione S-transferase subunits from Orthosia gothica.

Four subunits of the cytosolic glutathione S-transferase ( GST) in Orthosia gothica fed on willow leaves and a semisynthetic bean diet were purified as separate peaks (subunits 1-4) by a two-step gradient elution from a reverse-phase HPLC column after an initial purification by glutathione-Sepharose 1-chloro-2,4-dinitro-benzene (CDNB). Subunit 1 with a molecular weight of 26.0 kDa reconstituted into a GST homodimer with an isoelectric point of 4.8 and the N-terminal amino acid sequence (27 steps) indicated a relationship to the class theta GST of Musca domestica in the first 10 steps (50% homology), but also to the GST class pi of Caenohrabditis elegans (50% between steps 10 and 20). The three subunits 2-4 all had a molecular weight of 23.5 kDa and the isoelectric points of the reconstituted homodimers were > 9. 0. The N-terminal amino acid sequence was determined (24 steps) and was identical for the three subunits. A high identity of sequence to the GST in C. elegans (70% between steps 1 and 17), and a low homology (25%) to the O. gothica subunit 1 was observed. Thus, we suggest the O. gothica subunit 1 belong to a different class (O. gothica GST class 1) of GST than subunits 2-4 (O. gothica GST class 2). When the larvae hatched and fed on a semisynthetic bean diet, subunits 3 and 4 were not present in the HPLC eluate, and the subunit 2/subunit 1 ratio increased compared to the corresponding ratio in the larvae which hatched and fed on willow leaves until the third instar.[1]

References

The separation and identification of glutathione S-transferase subunits from Orthosia gothica. Egaas, E., Sandvik, M., Svendsen, N.O., Skaare, J.U. Insect Biochem. Mol. Biol. (1995) [Pubmed]

Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.