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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Cloning and characterization of cDNAs encoding oat PF1: a protein that binds to the PE1 region in the oat phytochrome A3 gene promoter.

In monocotyledons, the expression of the oat phytochrome A gene (PHYA) is down-regulated by phytochrome itself. This autoregulatory repression is the most rapid light-induced effect on gene expression reported in plants to date. A functional analysis of the oat PHYA3 gene minimal promoter in a rice transient expression assay has identified two promoter elements, PE1 and PE3, that interact synergistically in positive regulation. We have isolated an oat cDNA clone (pO2) that encodes a DNA-binding protein that binds to the PE1 region of the oat PHYA3 gene promoter. The in vitro binding properties of the pO2-encoded protein, towards DNA probes containing either the PE1 sequence or linker-substitution mutations in PE1, correlate with the activity of these DNA elements in the rice transient expression assay. These mutations are known to abolish expression of a reporter gene in vivo. Binding of these linker-substitution mutants to the pO2-encoded protein in vitro was lower by one to two orders of magnitude than the binding of the native PE1 region. We suggest, therefore, that the pO2 clone may encode the putative nuclear factor, oat PF1, that is involved in positive regulation of PHYA3 by binding to PE1 in vivo. pO2 encodes a 170-amino-acid-long protein that contains three repeats of the 'AT-hook' DNA-binding motif found in high mobility group I-Y (HMGI-Y) proteins. Oat PF1 is highly similar to rice PF1 and to the protein encoded by soybean cDNA SB16. They all have a strong similarity in their N-terminus to the pea H1 histone, and the presence of several AT-hook DNA-binding motifs in their C-terminal halves.[1]

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