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Terada, K. et al.

Construction of a plasmid for high level expression of Escherichia coli phosphoenolpyruvate carboxylase.

The minimum size DNA fragment (3011 bp) containing the entire phosphoenolpyruvate carboxylase [EC 4.1.1.31] gene of E. coli was cloned into a modified plasmid vector of high copy-number. The gene expression was directed by its own promoter and the content of the enzyme reached about 30% of total soluble protein of the transformed cells.[1]

References

Construction of a plasmid for high level expression of Escherichia coli phosphoenolpyruvate carboxylase. Terada, K., Fujita, N., Katsuki, H., Izui, K. Biosci. Biotechnol. Biochem. (1995) [Pubmed]

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