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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Characterisation of a haemolysin related to Vp- TDH produced by a Kanagawa phenomenon-negative clinical isolate of Vibrio parahaemolyticus.

The production of a family of haemolysins--thermostable direct haemolysin (Vp- TDH), Vp-TDH-related haemolysin (Vp- TRH) and Vp- TDH/I--has been reported in clinical isolates of Vibrio parahaemolyticus. This paper describes a fourth type of haemolysin--Vp-TDH/II--produced by a Kanagawa phenomenon-negative clinical isolate of V. parahaemolyticus (O13:K, untypable). Vp- TDH/II was purified by ammonium sulphate precipitation and successive filtrations on DEAE-cellulose, hydroxyapatite, Sepharose 4B and Mono Q columns. Vp- TDH/II was biophysicochemically and immunologically similar to, but not identical to Vp- TDH, Vp- TRH and Vp- TDH/I. Vp- TDH/II stimulated vascular permeability in rabbit skin and was lethal to mice. Purified Vp- TDH/II and viable cells of the Vp- TDH/II-producing strain both induced fluid accumulation in ligated rabbit intestine. The plasmid-determined structural gene for Vp- TDH/II was cloned and the nucleotide sequence determined. The deduced amino acid sequence of Vp- TDH/II differed from those of Vp- TDH, Vp- TRH and Vp- TDH/I.[1]

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