Disease relevance of Alveograf

• With respect to extractable estrogen receptor in the nucleus, 11/14 primary and 3/9 metastatic ovarian adenocarcinomas had greater than 50 fmol/g wet weight, as assayed by exchange at 30 degrees C for 5 hours after adsorption of the extracted receptor to hydroxylapatite [1].
• Calcium hydroxyapatite can be a significant component of black pigment gallstones [2].
• Particles of hydroxyapatite were identified in the joints of six patients diagnosed as having osteoarthritis, three of whom had acute inflammatory episodes with effusions into the joints [3].
• Samples of proliferative synovium, joint capsule, and cartilage from the patient with chondrocalcinosis showed abundant calcium oxalate crystals, and not calcium pyrophosphate dihydrate or calcium hydroxyapatite [4].
• Experimental injection of hydroxyapatite crystals into dog knee joints produces inflammation supporting the potential role for these crystals in joint disease [5].

Psychiatry related information on Alveograf

• Hydroxylapatite binding was used to follow the reaction time course [6].
• CONCLUSION: Use of bovine pericardium as wrapping material for the hydroxyapatite implants has shown promising results with minimal extrusion rates providing an effective alternative for sclera, eliminating the potential risks of CJD [7].
• Correlative radiological, self-assessment and clinical analysis of evolution in instrumented dorsal and lateral fusion for degenerative lumbar spine disease. Autograft versus coralline hydroxyapatite [8].

High impact information on Alveograf

• Osteonectin is a 32,000 dalton bone-specific protein that binds selectively to both hydroxyapatite and collagen [9].
• Two dyneins have been isolated from axonemes of Chlamydomonas flagella by a three step procedure consisting of extraction in a high salt containing buffer, hydroxyapatite chromatography and sedimentation in sucrose gradient [10].
• Using a procedure involving DEAE-cellulose, hydroxyapatite and gel filtration column chromatography, we obtained a fraction from human platelets which apparently can compete with 3H--cytochalasin B for binding to spectrin-actin complexes from human erythrocytes [11].
• Thermal stability was measured by hydroxyapatite thermal chromatography, S1 nuclease resistance after heating in a solvent which neutralizes the effect of DNA base composition, and spectrophotometric melting [12].
• Analysis of hybrids on hydroxyapatite in the presence of 8 M urea demonstrated that the poly(A) RNA complementary to repetitive DNA sequence components represented a population of molecules distinct from the population complementary to single-copy DNA [13].

Chemical compound and disease context of Alveograf

• The hydroxylapatite binding assay revealed average levels of 2 nmol of unoccupied 1,25-(OH)2D3 receptor per mg of protein in insect cells at 72 hr after infection with recombinant baculovirus [14].
• The main protein of the core of murine mammary tumor virus, with a molecular weight of 28,000 (p28), was solubilized by deoxycholate treatment of the virus and purified by Ultrogel ACA-54 filtration and hydroxyapatite chromatography [15].
• Of the major human bile salts, only the glycine-conjugated dihydroxy species prevent the transformation of amorphous calcium phosphate to calcium hydroxyapatite, a component of gallstones; we have proposed that this inhibition occurs by competition between the bile salt and HPO4(2-) anions for binding site on the apatite crystal embryo [16].
• Tropomyosin has been isolated from microvilli preparations from 13762 rat mammary adenocarcinoma ascites tumor cells by Triton extraction and pelleting of the microvillar microfilament core, extraction of the microfilament core with 1 M KCl, heat treatment, and hydroxyapatite chromatography [17].
• Nitric oxide reductase was purified from Paracoccus denitrificans very nearly to homogeneity by a simple method that involved the use of octyl glucoside to solubilize the enzyme from membranes and required a single hydroxyapatite column [18].

Biological context of Alveograf

• The kinetics of reassociation were studied by means of hydroxyapatite chromatography, single-strand-specific S1 nuclease, and optical methods of assay [19].
• These single copy sequences included about one third of the total nonrepetitive sequence in the genome as determined by the amounts recovered during the hydroxyapatite fractionation and by reassociation kinetics [20].
• To test this concept, single copy DNA sequences contiguous to interspersed repetitive sequences were prepared from sea urchin DNA by hydroxyapatite fractionation (repeat-contiguous DNA fraction) [20].
• The distribution of the middle repetitive sequences in the genome has been studied by an examination in the electron microscope of the structures obtained when middle repetitive sequences present on large DNA strands reassociate and by the hydroxyapatite binding methods developed by Davidson et al [21].
• The organization of three sequence classes--single copy, repetitive, and inverted repeated sequences--within the human genome has been studied by renaturation techniques, hydroxylapatite binding methods, and DNA hyperchromism [22].

Anatomical context of Alveograf

• It is double stranded, sediments at 7-8S in alkaline sucrose, and has a Tm determined optically and by thermal elution from hydroxyapatite that is substantially lower than that found for lymphocyte cell DNA [23].
• Hydroxyapatite was not detected in normal coronary artery sections [24].
• Complementary DNA (cDNA) specific for gamma-globin nucleotide sequences has been prepared by hybridizing total cDNA made from cord blood messenger RNA (mRNA) as template to an excess of normal adult human globin mRNA and recovering the single-stranded cDNA from hydroxylapatite [25].
• A method for identifying particles of crystalline calcium hydroxyapatite in synovial fluids and biopsy material has been developed with high-resolution scanning electron microscopy and an energy-dispersive micro-analytical system [3].
• To investigate interactions between cellular titin and myosin in vitro, we purified both proteins from isolated intestinal epithelial cell brush borders by a combination of gel filtration and hydroxyapatite column chromatography [26].

Associations of Alveograf with other chemical compounds

• Foldback DNA, isolated from renatured mouse main band DNA by hydroxyapatite chromatography, is spread for electron microscopy by the formamide isodenaturing technique [27].
• In the present study this factor has been purified to homogeneity using a sequence of eight purification steps (ammonium sulfate precipitation, ion exchange chromatography, molecular sieve chromatography, hydrophobic affinity chromatography, hydroxylapatite chromatography, fast protein liquid chromatography, and two HPLC steps) [28].
• In contrast, crystals of calcium pyrophosphate or of hydroxyapatite did not stimulate significant production of TNF or of message [29].
• To isolate the inhibitory factor from normal erythrocytes, membrane proteins were first extracted with butanol and then subjected to sequential anion exchange, hydroxylapatite, and hydrophobic chromatography [30].
• Glycochenodeoxycholic acid inhibits calcium phosphate precipitation in vitro by preventing the transformation of amorphous calcium phosphate to calcium hydroxyapatite [2].

Gene context of Alveograf

• Purification entails lysis in a low salt, sucrose buffer that contains ATP, chromatography on DEAE-cellulose, precipitation with actin in the absence of ATP, gel filtration in a discontinuous KI-KCl buffer system, and hydroxylapatite chromatography [31].
• CDF is first enriched by three successive chromatographic procedures that utilize anion exchange, hydroxyapatite, and phenyl-Superose [32].
• It encodes the mouse homolog of rat osteopontin, a 41.5-kDa glycosylated bone phosphoprotein that binds to fibroblasts and osteosarcoma cells and to hydroxylapatite (bone matrix) [33].
• Using a cell-free system in vitro, we evaluated the action of DMP1 in the regulation of hydroxylapatite (HA) formation and crystal growth [34].
• PC-1 also increased matrix calcification (with hydroxyapatite crystals) by meniscal cells [35].

Analytical, diagnostic and therapeutic context of Alveograf

• The protein has been purified to homogeneity by gel filtration and chromatography on columns of DEAE-cellulose and hydroxyapatite [36].
• Template-directed oligonucleotide ligation on hydroxylapatite [37].
• Distribution of hydroxyapatite coincided with a similar distribution of calcium detected by a radiodense pattern using contact microradiography of the same sections before cytochemical staining [24].
• Rat lingual lipase was purified to homogeneity by solubilization in 1% octylglucoside, followed by centrifugation, affinity chromatography on hydroxylapatite, gel filtration, and chromatofocusing [38].
• In the presence of retinal, detergent and salt the native protein was obtained in pure form by sucrose density gradient centrifugation, hydroxyapatite chromatography and gel filtration [39].

References