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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A survey to assess the ultrastructural preservation of fixed biological samples after air-drying from tetramethylsilane.

A scanning electron microscope (SEM) survey assessed the quality of ultrastructural preservation of a wide range of tissues from the laboratory rat and from a limited number of botanical and fly-larval samples, air-dried from tetramethylsilane (TMS) after chemical fixation and dehydration. Comparisons were made with critical point drying (CPD) and air-drying from acetone. CPD produced good results in all cases. TMS gave acceptable results with 75%, and acetone with 31%, of rat tissues. Air-drying was unacceptable for the botanical and fly-larval samples. It is concluded that air-drying from TMS is generally useful for mammalian tissues in preparation for the SEM.[1]

References

  1. A survey to assess the ultrastructural preservation of fixed biological samples after air-drying from tetramethylsilane. Reville, W.J., Heapes, M.M., O'Sullivan, V.R. Journal of electron microscopy. (1994) [Pubmed]
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