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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Expression of a yeast intron-containing tRNA in the archaeon Haloferax volcanii.

Expression of the yeast tRNAPro(UGG) gene in Haloferax volcanii resulted in the production of a single stable transcript that had not undergone intron processing or processing of 5' and 3' flanking sequences. Mutation of the exon-intron boundary region of this RNA to produce a precursor RNA with the preferred halobacterial consensus exon-intron boundary structure did not restore intron processing. Processing of 5' and 3' flanking sequences was restored when the acceptor stem U6-U67 pair was changed to A6-U67. The significance of these results in defining the recognition requirements of tRNA maturation enzymes in the halophilic domain Archaea is discussed.[1]


  1. Expression of a yeast intron-containing tRNA in the archaeon Haloferax volcanii. Palmer, J.R., Nieuwlandt, D.T., Daniels, C.J. J. Bacteriol. (1994) [Pubmed]
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