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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Three-way differentiation of Chinese hamster ovary chromosomes by immunoperoxidase technique using a monoclonal anti-bromodeoxyuridine antibody.

A new permanent staining procedure for sister chromatid differentiation (SCD) in cultured Chinese hamster ovary cells has been established by combining the three-way differentiation in third mitosis (M3) chromosomes and the immunoperoxidase reaction developed with 3,3'-diaminobenzidine using a monoclonal anti-bromodeoxyuridine (BrdU) antibody. This procedure allows SCD at very low BrdU concentrations, and the evaluation of the sister chromatid exchange frequencies on a per cell-cycle basis.[1]

References

  1. Three-way differentiation of Chinese hamster ovary chromosomes by immunoperoxidase technique using a monoclonal anti-bromodeoxyuridine antibody. Piñero, J., Ortiz, T., Cortés, F. Biotechnic & histochemistry : official publication of the Biological Stain Commission. (1993) [Pubmed]
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