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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Cryopreservation of Angiostrongylus cantonensis third-stage larvae.

An optimum protocol for the cryopreservation of third-stage larvae (L3) of Angiostrongylus cantonensis was determined by: (i) use of different dimethyl sulphoxide (DMSO) concentrations; (ii) use of different FBS concentrations in RPMI-1640 medium; and (iii) variation in the thawing temperature. Studies showed that with concentrations of DMSO at 4.8% and FBS at 40% and a thawing temperature of 43 degrees C, L3 survival assessed by motility was highest at 56%. The infectivity of the cryopreserved L3 of A. cantonensis was 20% compared to controls obtained from slugs.[1]


  1. Cryopreservation of Angiostrongylus cantonensis third-stage larvae. Mahajan, R.K., Renapurkar, D.M. J. Helminthol. (1993) [Pubmed]
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