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MeSH Review


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Disease relevance of Cryopreservation


High impact information on Cryopreservation

  • In wild-type mdr2 (+/+) mice, ultrarapid cryofixation of livers in situ revealed abundant unilamellar lipid vesicles within bile canalicular lumina [6].
  • Standardization of the chromium-51 release, cell-mediated cytotoxicity assay: cryopreservation of mouse effector and target cells [7].
  • We report that the introduction of low concentrations of intracellular trehalose can greatly improve the survival of mammalian cells during cryopreservation [8].
  • Cryopreservation in 5% dimethyl sulfoxide/6% pentastarch was associated with superior cell recovery (both SCT and control subjects) compared with 10% dimethyl sulfoxide (P =.001) [3].
  • Lethally irradiated Balb-C mice were transplanted with previously frozen syngeneic marrow mononuclear cells (10(6)/mouse), one tenth of which (10(5)) had been primed with [TPO, KL, IL-1a, and IL-3] under serum-free conditions for 36 hours before cryopreservation [9].

Chemical compound and disease context of Cryopreservation


Biological context of Cryopreservation

  • After a 4-h exposure with 75 micrograms/ml ET-18-OCH3 at a cell density of 2 x 10(5)/ml the number of colonies of HTB 19 decreased from 75 +/- 10/10(3) cells (100%) to 1 +/- 0/10(3) cells (1%), and after subsequent cryopreservation no remaining colonies were found [15].
  • Lamina-specific cell adhesion is independent of divalent cations, is abolished after cryofixation or paraformaldehyde fixation and is recognized across species [16].
  • Using this information, a cryopreservation method was developed and the cryosurvival and fetal development of Sprague-Dawley zygotes cryopreserved in either EG, DMSO, or PG and plunged at either -30 or -80 degrees C, were assessed [17].
  • Rapid-cooling cryopreservation protocols with high DMSO concentrations (3.5M, 25% v/v) resulted in low post-thaw cell viability (<10%), which did not improve with higher concentrations (4.5M, 32% v/v) and longer exposure to DMSO, even though the majority of microcapsules (60-80%) remained intact [18].
  • After cryopreservation, no differences were observed on embryo development, but a significant increase on embryo hatching was found between unsupplemented and supplemented oocytes with 100 and 50 microM of cysteamine during IVM and IVC, respectively (P < 0.05) [19].

Anatomical context of Cryopreservation


Associations of Cryopreservation with chemical compounds


Gene context of Cryopreservation

  • Only TIL grown with low dose IL-2 and autologous tumor maintained long-term (over 4 months in culture) specific cytotoxicity against the autologous tumor, even upon cryopreservation and regrowth [29].
  • Cryopreservation induced mRNA for selected growth factors, including vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) A chain, which increased 5- to 20- fold at 48 h returning to basal levels by 120 h [30].
  • Adhesion molecules on peripheral blood-derived CD34+ cells: effects of cryopreservation and short-term ex vivo incubation with serum and cytokines [31].
  • An increase of allergen and PHA stimulated IFN-gamma secretion in atopic children was found following cryopreservation, but no such increase in auto-antigen induced IFN-gamma was seen in MS-patients [32].
  • In contrast, cells activated before cryopreservation had a significantly lower cytotoxic activity and the number of cells expressing the IL-2 receptor was also significantly reduced [33].

Analytical, diagnostic and therapeutic context of Cryopreservation


  1. Successful hematopoietic reconstitution with transplantation of erythrocyte-depleted allogeneic human umbilical cord blood cells in a child with leukemia. Pahwa, R.N., Fleischer, A., Than, S., Good, R.A. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  2. Myeloma progenitors in the blood of patients with aggressive or minimal disease: engraftment and self-renewal of primary human myeloma in the bone marrow of NOD SCID mice. Pilarski, L.M., Hipperson, G., Seeberger, K., Pruski, E., Coupland, R.W., Belch, A.R. Blood (2000) [Pubmed]
  3. Mobilization, collection, and processing of peripheral blood stem cells in individuals with sickle cell trait. Kang, E.M., Areman, E.M., David-Ocampo, V., Fitzhugh, C., Link, M.E., Read, E.J., Leitman, S.F., Rodgers, G.P., Tisdale, J.F. Blood (2002) [Pubmed]
  4. Fertility preservation in breast cancer patients: a prospective controlled comparison of ovarian stimulation with tamoxifen and letrozole for embryo cryopreservation. Oktay, K., Buyuk, E., Libertella, N., Akar, M., Rosenwaks, Z. J. Clin. Oncol. (2005) [Pubmed]
  5. Growth characteristics of murine tumors: neuroblastoma, renal cell adenocarcinoma, Wistar/Furth Wilms' after storage in a cryopreservation system. Murphy, G.P., Williams, P.D. Oncology (1984) [Pubmed]
  6. Hepatic secretion of phospholipid vesicles in the mouse critically depends on mdr2 or MDR3 P-glycoprotein expression. Visualization by electron microscopy. Crawford, A.R., Smith, A.J., Hatch, V.C., Oude Elferink, R.P., Borst, P., Crawford, J.M. J. Clin. Invest. (1997) [Pubmed]
  7. Standardization of the chromium-51 release, cell-mediated cytotoxicity assay: cryopreservation of mouse effector and target cells. Holden, H.T., Oldham, R.K., Ortaldo, J.R., Herberman, R.B. J. Natl. Cancer Inst. (1977) [Pubmed]
  8. Intracellular trehalose improves the survival of cryopreserved mammalian cells. Eroglu, A., Russo, M.J., Bieganski, R., Fowler, A., Cheley, S., Bayley, H., Toner, M. Nat. Biotechnol. (2000) [Pubmed]
  9. In vitro and in vivo evidence that ex vivo cytokine priming of donor marrow cells may ameliorate posttransplant thrombocytopenia. Ratajczak, M.Z., Ratajczak, J., Machalinski, B., Mick, R., Gewirtz, A.M. Blood (1998) [Pubmed]
  10. Purging of peripheral blood stem cell transplants in AML: a predictive model based on minimal residual disease burden. Feller, N., Jansen-van der Weide, M.C., van der Pol, M.A., Westra, G.A., Ossenkoppele, G.J., Schuurhuis, G.J. Exp. Hematol. (2005) [Pubmed]
  11. Cryopreservation of all prezygotes in patients at risk of severe hyperstimulation does not eliminate the syndrome, but the chances of pregnancy are excellent with subsequent frozen-thaw transfers. Queenan, J.T., Veeck, L.L., Toner, J.P., Oehninger, S., Muasher, S.J. Hum. Reprod. (1997) [Pubmed]
  12. Fertility preservation in breast cancer patients: IVF and embryo cryopreservation after ovarian stimulation with tamoxifen. Oktay, K., Buyuk, E., Davis, O., Yermakova, I., Veeck, L., Rosenwaks, Z. Hum. Reprod. (2003) [Pubmed]
  13. Treatment of multiple myeloma with intensive chemotherapy followed by autologous BMT using marrow purged with 4-hydroperoxycyclophosphamide. Reece, D.E., Barnett, M.J., Connors, J.M., Klingemann, H.G., O'Reilly, S.E., Shepherd, J.D., Sutherland, H.J., Phillips, G.L. Bone Marrow Transplant. (1993) [Pubmed]
  14. Exposure to dimethyl sulfoxide at 37 degrees C prior to freezing significantly improves the recovery of cryopreserved hybridoma cells. Wewetzer, K., Dilmaghani, K. Cryobiology (2001) [Pubmed]
  15. Removal of breast cancer cells from bone marrow by in vitro purging with ether lipids and cryopreservation. Dietzfelbinger, H.F., Kühn, D., Zafferani, M., Hanauske, A.R., Rastetter, J.W., Berdel, W.E. Cancer Res. (1993) [Pubmed]
  16. Lamina-specific cell adhesion on living slices of hippocampus. Förster, E., Kaltschmidt, C., Deng, J., Cremer, H., Deller, T., Frotscher, M. Development (1998) [Pubmed]
  17. Cryobiology of rat embryos I: determination of zygote membrane permeability coefficients for water and cryoprotectants, their activation energies, and the development of improved cryopreservation methods. Pfaff, R.T., Agca, Y., Liu, J., Woods, E.J., Peter, A.T., Critser, J.K. Biol. Reprod. (2000) [Pubmed]
  18. Strategies for the cryopreservation of microencapsulated cells. Chin Heng, B., Yu, H., Chye Ng, S. Biotechnol. Bioeng. (2004) [Pubmed]
  19. Cysteamine supplementation during in vitro maturation and embryo culture: a useful tool for increasing the efficiency of bovine in vitro embryo production. de Matos, D.G., Herrera, C., Cortvrindt, R., Smitz, J., Van Soom, A., Nogueira, D., Pasqualini, R.S. Mol. Reprod. Dev. (2002) [Pubmed]
  20. Autologous bone marrow transplantation for high-grade lymphoid malignancy using melphalan/irradiation conditioning without marrow purging or cryopreservation. The Northern Regional Bone Marrow Transplant Group. Carey, P.J., Proctor, S.J., Taylor, P., Hamilton, P.J. Blood (1991) [Pubmed]
  21. Purging of acute myeloid leukemic cells by ether lipids and hyperthermia. Okamoto, S., Olson, A.C., Berdel, W.E., Vogler, W.R. Blood (1988) [Pubmed]
  22. Purging leukemic cells from simulated human remission marrow with alkyl-lysophospholipid. Okamoto, S., Olson, A.C., Vogler, W.R., Winton, E.F. Blood (1987) [Pubmed]
  23. Caspase inhibition reduces apoptotic death of cryopreserved porcine hepatocytes. Yagi, T., Hardin, J.A., Valenzuela, Y.M., Miyoshi, H., Gores, G.J., Nyberg, S.L. Hepatology (2001) [Pubmed]
  24. Long-term patency of vein grafts preserved in liquid nitrogen in dimethyl sulfoxide. Weber, T.R., Lindenauer, S.M., Dent, T.L., Allen, E., Salles, C.A., Weatherbee, L. Ann. Surg. (1976) [Pubmed]
  25. Cryopreservation of human fetal pancreas. Brown, J., Kemp, J.A., Hurt, S., Clark, W.R. Diabetes (1980) [Pubmed]
  26. Successful long-term cryopreservation and transplantation of human fetal pancreas. Hullett, D.A., Bethke, K.P., Landry, A.S., Leonard, D.K., Sollinger, H.W. Diabetes (1989) [Pubmed]
  27. Stability of transbilayer phospholipid asymmetry in viable ram sperm cells after cryotreatment. Müller, K., Pomorski, T., Müller, P., Herrmann, A. J. Cell. Sci. (1999) [Pubmed]
  28. Graft vascular function after transplantation of pancreatic islets. Jansson, L., Carlsson, P.O. Diabetologia (2002) [Pubmed]
  29. Autologous tumor-specific cytotoxicity of tumor-infiltrating lymphocytes derived from human renal cell carcinoma. Koo, A.S., Tso, C.L., Shimabukuro, T., Peyret, C., deKernion, J.B., Belldegrun, A. J. Immunother. (1991) [Pubmed]
  30. Comparison of the stress response to cryopreservation in monolayer and three-dimensional human fibroblast cultures: stress proteins, MAP kinases, and growth factor gene expression. Liu, K., Yang, Y., Mansbridge, J. Tissue engineering. (2000) [Pubmed]
  31. Adhesion molecules on peripheral blood-derived CD34+ cells: effects of cryopreservation and short-term ex vivo incubation with serum and cytokines. Koenigsmann, M.P., Koenigsmann, M., Notter, M., Neuloh, M., Mücke, C., Thiel, E., Berdel, W.E. Bone Marrow Transplant. (1998) [Pubmed]
  32. Effect of cryopreservation on expression of Th1 and Th2 cytokines in blood mononuclear cells from patients with different cytokine profiles, analysed with three common assays: an overall decrease of interleukin-4. Kvarnström, M., Jenmalm, M.C., Ekerfelt, C. Cryobiology (2004) [Pubmed]
  33. The influence of cryopreservation on activity and surface markers of lymphokine-activated killer cells. Letellier, C., Rameliarison, L., Fizet, D., Ferrer, A.M., Vezon, G. Vox Sang. (1991) [Pubmed]
  34. Collection of pluripotential hematopoietic stem cells by cytapheresis. Lasky, L.C., Ash, R.C., Kersey, J.H., Zanjani, E.D., McCullough, J. Blood (1982) [Pubmed]
  35. Allograft vein patency in a canine model. Additive effects of cryopreservation and cyclosporine. Augelli, N.V., Lupinetti, F.M., el Khatib, H., Sanofsky, S.J., Rossi, N.P. Transplantation (1991) [Pubmed]
  36. A combination of low-dose cyclophosphamide and colony-stimulating factors is more cost-effective than granulocyte-colony-stimulating factors alone in mobilizing peripheral blood stem and progenitor cells. Meisenberg, B., Brehm, T., Schmeckel, A., Miller, W., McMillan, R. Transfusion (1998) [Pubmed]
  37. A differential mechanism is involved during heparin- and cryopreservation-induced capacitation of bovine spermatozoa. Cormier, N., Bailey, J.L. Biol. Reprod. (2003) [Pubmed]
  38. Pronuclear stage cryopreservation after intracytoplasmic sperm injection and conventional IVF: implications for timing of the freeze. Damario, M.A., Hammitt, D.G., Galanits, T.M., Session, D.R., Dumesic, D.A. Fertil. Steril. (1999) [Pubmed]
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