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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A continuous spectrophotometric assay for the aminoacylation of transfer RNA by alanyl-transfer RNA synthetase.

A continuous spectrophotometric assay is described for the aminoacylation reaction catalyzed by Escherichia coli alanyl-transfer RNA synthetase. The assay is based on coupling the alanyl-tRNA synthetase-dependent formation of AMP to the lactate dehydrogenase oxidation of NADH. Oxidation of NADH, as monitored at 340 nm, is shown to be stoichiometric with the formation of alanyl-tRNA(Ala). This assay will facilitate the rapid accumulation and analysis of kinetic data for alanyl-tRNA synthetase and should be applicable to aminoacyl-tRNA synthetases in general.[1]

References

  1. A continuous spectrophotometric assay for the aminoacylation of transfer RNA by alanyl-transfer RNA synthetase. Wu, M.X., Hill, K.A. Anal. Biochem. (1993) [Pubmed]
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