Nucleosome-specific antibody from an autoimmune MRL/Mp-lpr/lpr mouse.
OBJECTIVE. To characterize the binding properties and variable-region sequences of LG4-1, a monoclonal antibody from an autoimmune MRL/Mp-lpr/lpr mouse that reacts specifically with nucleosome core particles and represents a new antinuclear antibody specificity. METHODS. The reactivity of the antibody against various nuclear substrates was determined using an enzymatic immunoassay, and the variable-region genes were sequenced from messenger RNA, using the dideoxy chain termination method. RESULTS. LG4-1 was found to react with nucleosome core particles but not with individual histones and DNA, or with various histone-histone and histone-DNA complexes. It was demonstrated that this antibody is encoded by a combination of variable-region genes and gene segments that have undergone few somatic mutations. CONCLUSION. The nucleosome core particle expresses a unique conformational autoepitope(s) resulting from the ordered association of histones and DNA.[1]References
- Nucleosome-specific antibody from an autoimmune MRL/Mp-lpr/lpr mouse. Losman, J.A., Fasy, T.M., Novick, K.E., Massa, M., Monestier, M. Arthritis Rheum. (1993) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
