Disease relevance of Antibody Specificity

• The antibody specificity of immunoglobulin G bound to the liver cell membrane during hepatitis B virus infection and chronic liver disease has been studied in 8 patients after antibody elution with high molar urea [1].
• The pattern of antibody specificities in sera from patients with myasthenia gravis (MG) was determined by the ability of monoclonal antibodies against defined determinants on the acetylcholine receptor molecule to inhibit binding of the serum antibodies to receptor from human muscle [2].
• OBJECTIVE: To identify a finer level of antibody specificity for risk of congenital heart block (CHB) than reactivity to 52-kd SSA/Ro (Ro 52) [3].
• These results were not consistent with a common melanoma antigen on human melanoma cells but rather indicated heterogeneity of melanoma antigens and multiple antibody specificities in the sera tested [4].
• At least four major groups of antibody specificities were identified: Salmonella (i) BO specific, (ii) CO specific, (iii) DO specific, and (iv) EO specific [5].

High impact information on Antibody Specificity

• We conclude with an analysis of the highly diverse CDR3 loops found in all antigen receptor molecules and suggest that such regions form the core of both TCR and antibody specificity [6].
• Ten of the 16 mothers with infected children (62 percent) acquired new antibody specificities against glycoprotein H, as compared with only 4 of the 30 mothers of uninfected infants (13 percent, P<0.001) [7].
• BRCA1 protein products: antibody specificity.. [8].
• Inheritance of antibody specificity. III. A new VH gene controls fine specificity of anti-p-azobenzenearsonate coupled to the carbon atom 5 of hydroxyphenylacetic acid in the mouse [9].
• In contrast to the other three antibody specificities, GADA levels were not related to the risk for IDDM [10].

Chemical compound and disease context of Antibody Specificity

• These findings demonstrate the heterogeneity of antiphospholipid antibody specificity induced in patients treated with various phenothiazine drugs and indicate that none of these patterns of reactivity marks a predisposition for thromboembolism in this population [11].
• The animals were challenged by intramammary infusion of either tetanus toxoid and lipopolysaccharide (LPS) or ovalbumin and LPS, and changes in the ratios of the two antibody specificities in mammary secretions were monitored for the first 8 h of the resulting acute inflammatory episode [12].
• The antibody specificity was determined by the inhibitory effects of the methyl glycosides of constituent mono- and oligosaccharides synthesized related to the O-antigenic polysaccharide from E. coli O128 [13].

Biological context of Antibody Specificity

• Differences were found for both the maximum number of sites immunoprecipitated by each antibody in one brain region and for the percentage of benzodiazepine binding sites immunoprecipitated by one specificity antibody between the different brain regions [14].
• Cross-reactions between 2, 4-dinitrophenyl and menadione (vitamin K3) and the general problem of antibody specificity [15].
• Only sera with antibody specificity to both N-terminal and basic functional domains were able to inhibit extracellular Tat-dependent transactivation significantly in vitro [16].
• Together, these data show that the mode of antigen presentation employed in IFN-beta ELISAs determines which antibody specificities are detected, and can affect whether or not a given serum sample is identified as positive for anti-IFN-beta antibodies [17].
• Inspection of the amino acid sequences of 136 HLA-A, B and C molecules allowed the prediction of five unique epitopes corresponding to these antibody specificities, a possible epitope unique to A2403 and confirmation of a likely epitope shared by A1 and A24 [18].

Anatomical context of Antibody Specificity

• Certain antibodies had the ability to increase HA binding by lymphocytes but this did not correlate absolutely with antibody specificity and was only partially attributable to CD44 cross-linking [19].
• An analysis of heavy chain glycopeptides of hybridoma antibodies: correlation between antibody specificity and sialic acid content [20].
• The frequencies of lipopolysaccharide- (LPS) reactive B cells and their antibody specificity repertoire have been determined in the spleen and bone marrow (BM) of mice at different ages [21].
• Anti-CII antibodies contained in RA SF represent one of many antibody specificities reacting with chondrocyte membrane antigens [22].
• This study has identified a novel islet cell antibody specificity and demonstrates that in islet cell antibody positive endocrine autoimmune patients, only islet cell antibodies which stain both Beta and Alpha cells are associated with progression to Type 1 diabetes [23].

Associations of Antibody Specificity with chemical compounds

• Antibodies to doxorubicin, which is both immunosuppressive and toxic, as well as a high affinity and high specificity antibody to the steroid hormone oestradiol have been isolated [24].
• We examined the effect of antibody specificity, concentration, and isotype on liposome-mediated drug delivery to AKR/J SL2 cells [25].
• Antibody specificity was characterized by using compounds chemically related to FdUrd and comparing their effectiveness for displacing [3H]FdUrd from the antibody-combining site [26].
• Antibody specificity was characterized by comparing the effectiveness of various related compounds to displace labeled methotrexate from the antibody-binding site [27].
• Antibody specificity was determined by autoradiographic detection of 32P labeled RNA which had been separated by polyacrylamide gel electrophoresis [28].

Gene context of Antibody Specificity

• Diversity in the CDR3 region of V(H) is sufficient for most antibody specificities [29].
• Western blotting was performed and showed high anti-COX-2 antibody specificity and the presence of COX-2 protein in the sample tissues [30].
• Monoclonal antibodies to carcinoembryonic antigen: a systematic analysis of antibody specificities by using related normal antigens and evidence for allotypic determinants on carcinoembryonic antigen [31].
• To characterize the binding properties and variable-region sequences of LG4-1, a monoclonal antibody from an autoimmune MRL/Mp-lpr/lpr mouse that reacts specifically with nucleosome core particles and represents a new antinuclear antibody specificity [32].
• The data further demonstrate that detection of cell surface gC1qR may be dependent on antibody specificity [33].

Analytical, diagnostic and therapeutic context of Antibody Specificity

• In immunoprecipitation assays, the purified anti-B" antibody specificity also reacts with U1 RNPs which is due to cross-reactivity of the antibody with the U1 RNA-specific A protein, as demonstrated by immunoblotting using proteins from isolated U1 RNPs as antigenic material [34].
• We have measured the antibody specificities to the two polysaccharides in sera from asymptomatic group C meningococcal carriers and vaccinated adults by a new enzyme-linked immunosorbent assay (ELISA) procedure using methylated human serum albumin for coating the group C polysaccharide onto microtiter plates [35].
• CT7-33 was effective in frozen as well as formalin-fixed, paraffin-embedded tissues, and immunohistochemistry/reverse transcriptase polymerase chain reaction (RT-PCR) co-typing demonstrated antibody specificity [36].
• The spectrum of Hib-directed antibody specificities detected in sera from immunized animals was essentially identical to that detected in bronchoalveolar lavage fluids from these same animals [37].
• Chimeric T cell receptors (cTCR) with an antibody specificity have been proposed in several models as a combination of antibody and cellular immunotherapy without MHC restriction [38].

References