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Gilligan, P.H. et al.

The use of a commercially available enzyme immunoassay for the detection of Clostridium difficile toxin A.

A commercially available enzyme immunoassay (EIA) for the detection of Clostridium difficile toxin A (Premier Toxin A EIA, Meridian Diagnostics, Cincinnati, Ohio) was compared with tissue culture cytotoxicity assay, enterotoxigenic culture, and latex agglutination test for the laboratory diagnosis of C difficile-associated disease. When evaluated for detection of C difficile-associated disease using clinical specimens, EIA was the most sensitive (83.1%) and tissue culture cytotoxicity assay was the most specific test with EIA, tissue culture cytotoxicity assay and enterotoxigenic culture having similar correlation values (96.6, 96.1, 94.0%, respectively). The latex agglutination test was not as accurate (89.7% correlation) as the other three tests due mainly to its poor sensitivity (47.9%). The EIA is a rapid, easy-to-use alternative to tissue culture cytotoxicity assay for detection of C difficile-associated disease.[1]

References

The use of a commercially available enzyme immunoassay for the detection of Clostridium difficile toxin A. Gilligan, P.H., Walden, T.P., Kelly, W.F., Wait, K.J., Kraft, J.A., Willis, D.H. Arch. Pathol. Lab. Med. (1993) [Pubmed]

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