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Ruiz-Vázquez, P. et al.

Structure of the phenylalanine hydroxylase gene in Drosophila melanogaster and evidence of alternative promoter usage.

The complete Drosophila melanogaster phenylalanine hydroxylase gene isolated from a genomic library was sequenced. Gene structure consisted of five exons covering a region of around 3 kb. Position of introns in the C-terminal domain was conserved with mammalian aromatic amino acid hydroxylase genes. Putative promoter sequences in the 5'UTR and intron 1 were identified. A novel transcript was detected differing from that previously reported by the inclusion of a part of the intron 1 sequence. It could be produced using an alternative promoter. The deduced open reading frame would code a protein with a small difference at the N-terminus. Expression of the alternative transcripts was examined throughout development.[1]

References

Structure of the phenylalanine hydroxylase gene in Drosophila melanogaster and evidence of alternative promoter usage. Ruiz-Vázquez, P., Moulard, M., Silva, F.J. Biochem. Biophys. Res. Commun. (1996) [Pubmed]

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