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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

The isolation of a suppressible nonsense mutant in mammalian cells.

An HGPRT- cell line derived from mouse L cells has been shown to have the following properties: it is CRM+; the defective HGPRT molecules are altered in the carboxyterminal peptide; the mutant cells regain HGPRT activity when ochre-suppressor tRNA is microinjected into them, but not when amber-suppressor or wild-type tRNAs are injected. We conclude from these properties that this mutant cell line contains an ochre nonsense mutation (UAA) in the structural gene for HGPRT.[1]

References

  1. The isolation of a suppressible nonsense mutant in mammalian cells. Capecchi, M.R., Haar, R.A., Capecchi, N.E., Sveda, M.M. Cell (1977) [Pubmed]
 
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