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Gene Review

Och  -  ochre

Mus musculus

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High impact information on Och

  • We conclude from these properties that this mutant cell line contains an ochre nonsense mutation (UAA) in the structural gene for HGPRT [1].
  • Within a 67-base-pair exon of the rd PDE beta-subunit gene, we identified a nonsense ochre mutation (a C----A transversion in codon 347) that truncates the normal gene product, eliminating more than one-half of the peptide chain, including the putative catalytic domain [2].
  • Multicopy tRNA expression plasmids were directly injected into skeletal muscle and into the hearts of transgenic mice carrying a reporter gene with an ochre mutation [3].
  • The product of suppression (chloramphenicol acetyltransferase) accumulates linearly with increases in suppressor tRNA concentration to the point where the ochre-suppressing tRNA(Ser) is in four- to fivefold excess over the endogenous tRNA(Ser) [3].
  • Double-site immunoassay and P. falciparum transient transfection using the reporter luciferase gene demonstrated translation through the internal ochre codon [4].

Biological context of Och


Anatomical context of Och

  • The wild-type amber (UAG) and in vitro-generated ochre (UAA) readthrough codon derivatives replicated in protoplasts [7].
  • The scFv gene in one of these clones was reamplified to introduce the ochre codons, and then expressed in the bacterial periplasm as the soluble antibody fragment [8].

Associations of Och with chemical compounds

  • On account of the spontaneous oxidation process, the colour of PPD increases and becomes ochre-brown in a few days, showing an absorption peak at lambda = 510 nm with shoulder at about 440 to 460 nm [9].


  1. The isolation of a suppressible nonsense mutant in mammalian cells. Capecchi, M.R., Haar, R.A., Capecchi, N.E., Sveda, M.M. Cell (1977) [Pubmed]
  2. Identification of a nonsense mutation in the rod photoreceptor cGMP phosphodiesterase beta-subunit gene of the rd mouse. Pittler, S.J., Baehr, W. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  3. Suppression of nonsense mutations in cell culture and mice by multimerized suppressor tRNA genes. Buvoli, M., Buvoli, A., Leinwand, L.A. Mol. Cell. Biol. (2000) [Pubmed]
  4. A member of the Plasmodium falciparum Pf60 multigene family codes for a nuclear protein expressed by readthrough of an internal stop codon. Bischoff, E., Guillotte, M., Mercereau-Puijalon, O., Bonnefoy, S. Mol. Microbiol. (2000) [Pubmed]
  5. Nucleotide sequence and evolution of the rat mitochondrial cytochrome b gene containing the ochre termination codon. Koike, K., Kobayashi, M., Yaginuma, K., Taira, M., Yoshida, E., Imai, M. Gene (1982) [Pubmed]
  6. Ochre suppressor transfer RNA restored dystrophin expression in mdx mice. Li, K., Zhang, J., Buvoli, M., Yan, X.D., Leinwand, L., He, H. Life Sci. (1997) [Pubmed]
  7. The tomato bushy stunt virus replicase proteins are coordinately expressed and membrane associated. Scholthof, K.B., Scholthof, H.B., Jackson, A.O. Virology (1995) [Pubmed]
  8. Single-chain Fv fragments derived from an anti-11-deoxycortisol antibody. Affinity, specificity, and idiotype analysis. Kobayashi, N., Shibahara, K., Ikegashira, K., Shibusawa, K., Goto, J. Steroids (2002) [Pubmed]
  9. Oxidized p-phenylenediamine: observations on the staining reaction in epoxy embedded tissues. Armas-Portela, R., Stockert, J.C., Ferrer, J.M., Tato, A., Gómez-Perretta, C., Callaghan, R.C. Acta Histochem. Suppl. (1986) [Pubmed]
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