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Structural organization and chromosomal assignment of the swine endothelin-1 gene.

Cosmid clone containing swine endothelin-1 (EDN1) gene, cosEDN1, was isolated from swine cosmid library using swine EDN1 cDNA as a probe. The sequence analysis of cosEDN1 DNA revealed that the swine EDN1 gene consists of 5 exons, spanning approximately 6.5 kb. In the 5'-upstream region of the EDN1 gene, AP-1 and NF-1 elements were found, suggesting the possibility that the expression of swine EDN1 gene is controlled by protooncogene products Fos and Jun, and TGF-beta. Fluorescence in situ hybridization (FISH) using cosEDN1 DNA as a probe demonstrated that EDN1 gene resides on swine chromosome 7p13- > pter.[1]

References

  1. Structural organization and chromosomal assignment of the swine endothelin-1 gene. Kojima, M., Ohata, K., Yasue, H. J. Vet. Med. Sci. (1997) [Pubmed]
 
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