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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Sub- and supercritical fluid extraction of trichloropyridinol from soil prior to immunoassay.

A comparative study on the extraction of TCP (3,5,6-trichloro-2-pyridinol, a metabolite of chlorpyrifos) from soil with CO2 and H2O is reported. The polarity of the analyte requires the presence of both a cosolvent (methanol) and an ion-pair reagent [(1R)-(-)-10-camphorsulfonic acid ammonium salt] for 95% extraction in 30 min when supercritical CO2 at 40 degrees C and 383 bar is used as extractant. Subcritical water (250 degrees C and 200 bar) enables complete extraction within 15 min without additives. Quantitation of the target analyte is performed by specific immunoassay using a non-commercial monoclonal antibody which provides a linear determination range between 0.005 and 5 micrograms/g, with coefficients of variation of 5.3 and 4.9% for the SC-CO2 and sub-H2O extractions, respectively.[1]

References

  1. Sub- and supercritical fluid extraction of trichloropyridinol from soil prior to immunoassay. Jiménez-Carmona, M.M., Manclús, J.J., Montoya, A., Luque de Castro, M.D. Journal of chromatography. A. (1997) [Pubmed]
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