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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Isolation and preliminary characterization of the Streptococcus mutans rpsJ gene.

We previously reported that four putative open reading frames were identified in the regions flanking the Streptococcus mutans GS-5 fructosyltransferase gene. For one of these, ORF 4, only a small region had been isolated and the first 30 nucleotides had been sequenced. In order to determine whether this open reading frame is part of an expressed gene, we isolated a DNA fragment containing intact ORF 4 and a portion of the downstream ORF 5 by inverse polymerase chain reaction. A comparison of the deduced amino acid sequences of ORF 4 and ORF 5 with other proteins revealed that the ORF 4 and ORF 5 gene products were highly homologous to ribosomal proteins S10 and L3, respectively, of several bacteria. To identify the precise transcriptional start site for the ORF 4 gene, primer extension analysis was carried out. The results indicated initiation at a G residue with corresponding -10 and -35 regions homologous to the Escherichia coli consensus promoter sequences. These results indicate that the sequences of ORF 4 and ORF 5 are consistent with the structures of ribosomal proteins S10 and L3, respectively, and are present in a ribosomal protein operon.[1]

References

  1. Isolation and preliminary characterization of the Streptococcus mutans rpsJ gene. Shibata, Y., Kuramitsu, H.K. Oral Microbiol. Immunol. (1996) [Pubmed]
 
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