Disease relevance of UNQ473

• Demeclocycline (7-chloro-6 demethyl tetracycline) (DMC) corrected the CBZ-induced water intoxication in a 51-year-old man with refractory complex partial seizures and a normal antidiuretic hormone (ADH) level [1].
• Here we report the antitumor effects of DMC in vivo, using a solid human tumor xenograft mouse model using human liver cancer SMMC-7721 cells [2].
• Outcome parameters of local control (LC), freedom from distant metastases (DMC), disease-free survival (DFS) and cancer specific survival (CSS) were evaluated relative to primary tumor characteristics [3].
• By quantitative real time RT-PCR 71% of breast tumors showed 3- to 24-fold up-regulation of VCC-1 [4].

High impact information on UNQ473

• In this study, we report the identification and characterization of a novel cytokine-like protein, DMC (dendritic cell and monocyte chemokine-like protein), which attracts dendritic cells and monocytes [5].
• Expression studies show that DMC is constitutively expressed in lung, suggesting a potential role for DMC in recruiting monocytes and dendritic cells from blood into lung parenchyma [5].
• Interestingly, DMC was able to initiate apoptosis via a p53-independent pathway whereas MC was not [6].
• The cytotoxicity of DMC parallels closely that of MC in a number of rodent cell lines tested, whereas 2,7-DAM is relatively noncytotoxic [6].
• In this report, we show that chromosomes with DRT/DMC contain phosphorylated Chk1, consistent with activation of the S-M phase checkpoint [7].

Biological context of UNQ473

• Furthermore, MC and DMC, but not 2,7-DAM, were able to induce apoptosis efficiently in ML-1 cells [6].
• Certain chromosome rearrangements display a significant delay in chromosome replication timing (DRT) that is associated with a subsequent delay in mitotic chromosome condensation (DMC) [7].
• Furthermore, we show that INCENP is recruited to the DRT/DMC chromosomes during all phases of mitosis [7].
• In contrast, Aurora B kinase is absent on DRT/DMC chromosomes when these chromosomes lack serine 10 phosphorylation of H3 [7].
• RESEARCH DESIGN AND METHODS: Diabetic patients who receive care in a county public health clinic were randomly selected for a Diabetes Managed Care Program (DMCP) in which a specially trained nurse followed detailed treatment algorithms to provide diabetes care for 1 year [8].

Anatomical context of UNQ473

• Consistent with our predictions, DMC induces migration of monocytes and immature dendritic cells [5].
• Finally, cells with DRT/DMC chromosomes have centrosome amplification, abnormal spindle assembly, endoreduplication and significant chromosome instability.Oncogene (2007) 26, 1852-1861. doi:10.1038/sj.onc.1209995; published online 25 September 2006 [7].
• Murine VCC-1 expression can also be detected in lung as well as thyroid, submaxillary gland, epididymis, and uterus tissues by slot blot analysis [4].
• In vitro, human microvascular endothelial cells grown on fibronectin increase VCC-1 expression by almost 100-fold [4].
• On the contrary, in granulocytes, the energy transfer from K-68 was 2.2 times more efficient than from the lipid-immersed DMC [9].

Associations of UNQ473 with chemical compounds

• DMC inhibits ADH-sensitive adenylate cyclase activity in the renal collecting duct and may be useful in correcting the ADH-like or renal antidiuretic effect of CBZ [1].
• [2-11C]Dimethyl carbonate (DMC) was prepared from [C-11]phosgene and excess of sodium methoxide in methanol containing dimethyl carbonate as added carrier [10].
• This involved the total syntheses of the terminal alkyne 33-O-Me-45,46-dehydrobromophorboxazole A (MDHBPA) and a terminal vinyl iodide derivative of the blue fluorescent dye N,N,-dimethyl-7-aminocoumarin (DMC) [11].
• Fifty-one percent met the ADA low-density lipoprotein cholesterol goal at entry into the DMCP compared with 82% at the end of the year [12].
• Transesterification conversion efficiencies of N-methylcarbamates dioxacarb, carbofuran and OMS-22, calculated from the amount of the on-column produced O-methyl-N-methylcarbamate (DMC), were 96, 77 and 76% with detection limits of 8, 10 and 10 ng, respectively [13].

Analytical, diagnostic and therapeutic context of UNQ473

• RESULTS: Use of the urgent care/emergency room and hospitalizations during the intervention year and the year prior were available for 331 patients who completed the DMCP intervention [8].
• The average tumor weights in the control group and in mice injected with 150 mg/kg DMC were 1.42+/-0.11 g and 0.59+/-0.12 g, respectively [2].

References