Disease relevance of HDAC11

• We have demonstrated expression of proinflammatory cytokines and chemokines in response to Campylobacter infection in avian primary chick kidney cells and the avian macrophage cell line HD11 [1].
• PURPOSE: To guarantee the treatment quality of involved-field radiotherapy (IF-RT) of patients in the Hodgkin's disease (HD)10 and HD11 trials of the German Hodgkin Study Group, with 460 participating study centers, a quality assurance program was conducted [2].
• The HD11 antibody at a concentration of 1 microgram/ml completely protected against the T-2-induced cytotoxicity of the human epidermoid carcinoma cell lines Hep-2 and KB [3].
• Intracellular killing of Salmonella enteritidis (SE) was also increased in ODN 2006-activated HD11 cells [4].

High impact information on HDAC11

• Epitope-tagged HDAC11 protein expressed in mammalian cells displays histone deacetylase activity in vitro [5].
• The predicted HDAC11 amino acid sequence reveals an open reading frame of 347 residues with a corresponding molecular mass of 39 kDa [5].
• Epitope-tagged HDAC11 protein localizes predominantly to the cell nucleus [5].
• Multiple tissue Northern blot and real-time PCR experiments show that the high expression level of HDAC11 transcripts is limited to kidney, heart, brain, skeletal muscle, and testis [5].
• Sequence analyses of the putative HDAC11 protein indicate that it contains conserved residues in the catalytic core regions shared by both class I and II mammalian HDAC enzymes [5].

Chemical compound and disease context of HDAC11

• The in vitro cytotoxicity of T-2, as measured by the inhibition of radiolabeled leucine uptake of the human epidermoid carcinoma Hep-2 and KB cell lines, was completely reversed by the addition of HD11 [6].

Biological context of HDAC11

• Determination of the exon-intron splice junctions established that HDAC11 is encoded by 9 exons ranging in size from 43 bp (exon 4) to 867 bp (exon 9) [7].
• Characterization of the 5' flanking genomic region, which precedes the HDAC11 open reading frame, revealed a TATA and CCAAT box-less promoter that contains a 1-kb CpG island [7].
• Fluorescence in situ hybridization analysis localized the human HDAC11 gene to chromosome 3p25, a region characterized by frequent gains and losses of chromosomal material in a number of various types of cancer [7].
• Mutational analyses of WNT7A and HDAC11 as candidate tumour suppressor genes in sporadic malignant pancreatic endocrine tumours [8].
• Results No point mutations, deletion or insertions were detected in either WNT7A or HDAC11 in any of the PETs [8].

Anatomical context of HDAC11

• We have isolated a novel 75-kDa gelatinase from a chicken macrophage cell line, HD11 [9].
• On a per cell basis, the activity of HD-11 cell 25-OHD3 1-hydroxylation reaction was comparable to that in primary cultures of chick renal tubular epithelial cells, which express the authentic renal 25-OHD3 1-hydroxylase [10].
• In these experiments we employed inducible nitric oxide synthase (iNOS)-free, intact mitochondria preparations from the avain macrophage-like cell line HD-11, which constitutively express the 1-hydroxylase, and nonenzymatically-generated NO to investigate NO-mediated autoregulation of the macrophage 1-hydroxylase [11].
• This study demonstrates the effects of recombinant human tumour necrosis factor a (rhTNF-alpha) and conditioned medium of the HD11-transformed chicken macrophage cell line on cultured chicken granulosa cells [12].
• Upon induction with lipopolysaccharide (LPS) the chicken macrophage cell line HD-11 secretes an activity that stimulates the synthesis of a CXC chemokine in the chicken fibroblast cell line CEC-32 [13].

Associations of HDAC11 with chemical compounds

• Subcellular localization and partial purification of the 25-hydroxyvitamin D3 1-hydroxylation reaction in the chick myelomonocytic cell line HD-11 [10].
• The recent identification of an immortalized chick myelomonocytic cell line, HD-11, that constitutively expresses a 25-hydroxyvitamin D (25-OHD) 1-hydroxylation reaction has alleviated dependence on studying primary macrophage cultures with no replicative potential in vitro [10].
• The HD11 trial compared four cycles of ABVD with four cycles of BEACOPP (bleomycin, etoposide, doxorubicin, cyclophosphamide, vincristine, procarbazine, and prednisone) baseline followed by 20 Gy IF-RT vs. 30 Gy IF-RT in a four-arm design [2].
• We have identified three novel chicken CC chemokine genes among cDNA clones derived from lipopolysaccharide-stimulated cells of the chicken macrophage cell line HD11 [14].
• HD11 anti-T-2 mAb, which bound to the T-2 metabolite acetyl T-2 (with a substituted acetyl group at R1 position), efficiently neutralized its in vitro cytotoxicity [3].

Other interactions of HDAC11

• We have cloned and characterized a human cDNA that belongs to the histone deacetylase family, which we designate as HDAC11 [5].
• We have analysed whether WNT7A or HDAC11 was biallelically inactivated in a group of well-characterized PETs [8].
• Randomized studies and most recently the GHSG HD 10 and HD 11 documented excellent results with low-dose IFRT of only 20 Gy in both early stage and in intermediate-stage patients [15].
• In this study, the authors report the case of a woman with anaplastic-lymphoma-kinase (ALK)-negative CD30-positive anaplastic large cell lymphoma, whose brother had developed HD 11 years previously [16].

Analytical, diagnostic and therapeutic context of HDAC11

• The T-2 detection limit in an enzyme-linked immunosorbent assay with HD11 was in the nanogram range [6].
• The central prospective radiation oncological review of all diagnostic imaging showed that corrections of disease involvement in 49% of patients with early stages (HD10) and in 67% for patients with intermediate stages (HD11) were necessary [17].

References