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Gene Review

MSP1  -  Msp1p

Saccharomyces cerevisiae S288c

 
 
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Disease relevance of MSP1

  • MATERIALS AND METHODS: Two recombinant proteins from the carboxy-terminus of MSP1, the 42 kD fused to GST (bMSP1(42)) and the 19 kD (yMSP1(19)), were expressed in Escherichia coli and secreted from Saccharomyces cerevisiae, respectively [1].
 

High impact information on MSP1

  • The mechanism of immunity is unclear, although data show that cell-mediated immunity plays a critical role following immunization with the larger mature MSP1 protein [2].
  • The MSP1 gene is not essential for the cell growth either on fermentable or nonfermentable carbon sources [3].
  • Intramitochondrial protein sorting. Isolation and characterization of the yeast MSP1 gene which belongs to a novel family of putative ATPases [3].
  • MSP1 was found to be an intrinsic mitochondrial outer membrane protein of an apparent molecular mass of 40 kDa with a large domain facing to the cytosol [3].
  • Further sequence analyses revealed that MSP1 is a member of a novel family of putative ATPases which share a highly conserved domain of about 185 amino acid residues, including a consensus motif for a nucleotide binding site [3].
 

Biological context of MSP1

 

Anatomical context of MSP1

  • P30P2MSP1(19) consists of two universal T-cell epitopes fused to the most C-terminal 19-kDa portion of MSP1, and this protein has previously shown promising potential as a vaccine for malaria [6].
 

Associations of MSP1 with chemical compounds

  • We isolated a novel yeast gene MSP1 (mitochondrial sorting of proteins) whose overexpression causes mislocalization of the 61mC1 fusion protein to the inner membrane, probably via the intermembrane space, and thereby allows the host cells to grow on glycerol [3].
  • Four antigenic variants of the 19-kDa carboxy terminal fragment of Plasmodium falciparum merozoite surface protein, MSP1 (MSP1(19)), were expressed in Saccharomyces cerevisiae as a histidine-tagged, secreted polypeptides (rMSP1(19)s) [7].
 

Other interactions of MSP1

  • A 795 bp region of Py17XL MSP-1 (bait), homologous to the Plasmodium falciparum MSP1(33) fragment, was inserted into a Gal4p DNA binding domain vector and used to screen the activation domain library (target) [8].
  • In vitro binding experiments confirmed the interaction between MSRP-1, MSRP-2, and the amino-terminal region of P. falciparum MSP-1 [5].
 

Analytical, diagnostic and therapeutic context of MSP1

  • In the MSP1 and control groups, protection correlated with antibody to MSP3 raised by challenge infection [9].
  • Immunofluorescence studies colocalized P. yoelii MSP-7 and P. yoelii MSRP-2 with the amino-terminal portion of MSP-1 and with each other on the surface of schizonts [10].

References

  1. Immunogenicity and in vivo efficacy of recombinant Plasmodium falciparum merozoite surface protein-1 in Aotus monkeys. Kumar, S., Yadava, A., Keister, D.B., Tian, J.H., Ohl, M., Perdue-Greenfield, K.A., Miller, L.H., Kaslow, D.C. Mol. Med. (1995) [Pubmed]
  2. Complete protective immunity induced in mice by immunization with the 19-kilodalton carboxyl-terminal fragment of the merozoite surface protein-1 (MSP1[19]) of Plasmodium yoelii expressed in Saccharomyces cerevisiae: correlation of protection with antigen-specific antibody titer, but not with effector CD4+ T cells. Hirunpetcharat, C., Tian, J.H., Kaslow, D.C., van Rooijen, N., Kumar, S., Berzofsky, J.A., Miller, L.H., Good, M.F. J. Immunol. (1997) [Pubmed]
  3. Intramitochondrial protein sorting. Isolation and characterization of the yeast MSP1 gene which belongs to a novel family of putative ATPases. Nakai, M., Endo, T., Hase, T., Matsubara, H. J. Biol. Chem. (1993) [Pubmed]
  4. A metalloproteinase gene from the pathogenic piscine hemoflagellate, Cryptobia salmositica. Jesudhasan, P.R., Tan, C.W., Woo, P.T. Parasitol. Res. (2007) [Pubmed]
  5. A multigene family that interacts with the amino terminus of plasmodium MSP-1 identified using the yeast two-hybrid system. Mello, K., Daly, T.M., Morrisey, J., Vaidya, A.B., Long, C.A., Bergman, L.W. Eukaryotic Cell (2002) [Pubmed]
  6. High-level production and purification of P30P2MSP1(19), an important vaccine antigen for malaria, expressed in the methylotropic yeast Pichia pastoris. Brady, C.P., Shimp, R.L., Miles, A.P., Whitmore, M., Stowers, A.W. Protein Expr. Purif. (2001) [Pubmed]
  7. Expression and antigenicity of Plasmodium falciparum major merozoite surface protein (MSP1(19)) variants secreted from Saccharomyces cerevisiae. Kaslow, D.C., Hui, G., Kumar, S. Mol. Biochem. Parasitol. (1994) [Pubmed]
  8. Interaction between two domains of the P. yoelii MSP-1 protein detected using the yeast two-hybrid system. Daly, T.M., Long, C.A., Bergman, L.W. Mol. Biochem. Parasitol. (2001) [Pubmed]
  9. Merozoite surface protein 3 and protection against malaria in Aotus nancymai monkeys. Hisaeda, H., Saul, A., Reece, J.J., Kennedy, M.C., Long, C.A., Miller, L.H., Stowers, A.W. J. Infect. Dis. (2002) [Pubmed]
  10. Members of the merozoite surface protein 7 family with similar expression patterns differ in ability to protect against Plasmodium yoelii malaria. Mello, K., Daly, T.M., Long, C.A., Burns, J.M., Bergman, L.W. Infect. Immun. (2004) [Pubmed]
 
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